Annapoorani Ponnambalam, Dhandapany Perundurai S, Sadayappan Sakthivel, Ramasamy Subbiah, Rathinavel Andiappan, Selvam Govindan Sadasivam
Department of Biochemistry, School of Biological Sciences, Madurai Kamaraj University, Madurai 625021, Tamil Nadu, India.
Atherosclerosis. 2006 May;186(1):173-6. doi: 10.1016/j.atherosclerosis.2005.07.004. Epub 2005 Aug 15.
Cardiac isoform of alpha-2 macroglobulin [cardiac alpha2M] has been shown to be an early marker in cardiac hypertrophy and left ventricular mass in humans. We, here, for the first time report its presence in myocardial infarcted humans and tried to explore the possibility of using this protein as a novel diagnostic marker for myocardial infarcted diabetic patients. A total of 260 samples were analyzed in this study for the presence of cardiac alpha2M. These include 55 patients of diabetic with post myocardial infarction [PMI], 45 diabetic patients without PMI, 60 patients of PMI alone and 100 controls without any ailments. Levels of cardiac alpha2M present in the sera of diabetic patients with PMI are significantly higher than that of normal human sera and diabetic patients without PMI but not with PMI alone group, suggesting this protein as a marker for PMI itself. However, our results reveal that cardiac alpha2M could be a valuable marker for the diagnosis of myocardial infarcted diabetic patients and differentiating them from diabetic patients without myocardial infarction by sandwich ELISA.
α-2巨球蛋白的心脏异构体[心脏α2M]已被证明是人类心脏肥大和左心室质量的早期标志物。在此，我们首次报告其在心肌梗死患者中的存在，并试图探索将这种蛋白质用作心肌梗死糖尿病患者新型诊断标志物的可能性。本研究共分析了260份样本以检测心脏α2M的存在。这些样本包括55例糖尿病合并心肌梗死后[PMI]患者、45例无PMI的糖尿病患者、60例仅患有PMI的患者和100例无任何疾病的对照者。患有PMI的糖尿病患者血清中存在的心脏α2M水平显著高于正常人血清和无PMI的糖尿病患者，但不高于仅患有PMI的组，这表明这种蛋白质是PMI本身的标志物。然而，我们的结果显示，心脏α2M可能是诊断心肌梗死糖尿病患者并通过夹心ELISA将他们与无心肌梗死的糖尿病患者区分开来的有价值的标志物。
