Ong Corinne S, Li Anna S, Priest Jeffrey W, Copes Ray, Khan Mohamad, Fyfe Murray W, Marion Stephen A, Roberts Jacquelin M, Lammie Patrick J, Isaac-Renton Judith L
Department of Pathology and Laboratory Medicine and Department of Health Care and Epidemiology, University of British Columbia, Vancouver, British Columbia, Canada.
Am J Trop Med Hyg. 2005 Aug;73(2):288-95.
A newly developed enzyme-linked immunosorbent assay (ELISA) that detects immunoglobulin G antibodies to the 27-kDa Cryptosporidium parvum sporozoite surface antigen was used to test 4,097 sera collected from pregnant women in 6 communities in British Columbia, Canada, between January 1996, and December 1997. Waterborne outbreaks of cryptosporidiosis occurred in two of the study communities during the period of follow-up, and ELISA seropositivity was high in all six communities during the study period (77% positive to 92% positive). In the community with the largest outbreak, levels of antibody to the 27-kDa antigen increased rapidly and then decayed to background levels within 3-4 months of the peak of the epidemic curve. Trends in serologic reactivity were complex in all communities, and increased antibody levels not related temporally to known waterborne outbreaks were also observed. Serological assays may provide more accurate information regarding community levels of Cryptosporidium infection.
一种新开发的酶联免疫吸附测定法（ELISA），用于检测针对27 kDa微小隐孢子虫子孢子表面抗原的免疫球蛋白G抗体，该方法被用于检测1996年1月至1997年12月期间从加拿大不列颠哥伦比亚省6个社区的孕妇中采集的4097份血清。在随访期间，两个研究社区发生了水源性隐孢子虫病暴发，并且在研究期间所有六个社区的ELISA血清阳性率都很高（77%阳性至92%阳性）。在暴发规模最大的社区，针对27 kDa抗原的抗体水平迅速上升，然后在疫情曲线峰值后的3 - 4个月内降至背景水平。所有社区的血清学反应趋势都很复杂，并且还观察到抗体水平升高与已知的水源性暴发在时间上无关。血清学检测可能会提供有关社区隐孢子虫感染水平的更准确信息。
