Department of Medical Microbiology and Parasitology, Shanghai Medical College of Fudan University , Shanghai, China.
Shanghai Key Laboratory of Atmospheric Particle Pollution Prevention (LAP3), Department of Environment Science and Engineering, Fudan University , Shanghai, China.
Biomicrofluidics. 2015 Apr 14;9(2):024114. doi: 10.1063/1.4916229. eCollection 2015 Mar.
Cryptosporidiosis has been reported to be associated with HIV/acquired immune deficiency syndrome, which greatly reduces the quality of life and shortens the life expectancy of HIV-infected patients. In order to properly treat the infected patients, accurate and automatic diagnostic tools need to be developed. In this study, a novel microfluidic immunochip system was presented for the surveillance and the rapid detection of Cryptosporidium infection in 190 HIV-infected patients from Guangxi, China, using the P23 antigen of Cryptosporidium. The procedure of detection can be completed within 10 min with 2 μl sample consumption. The system also was evaluated using the standard ELISA method. Among 190 HIV-infected individuals, the rate of P23 positivity was 13.7%. Seropositivity in HIV-infected individuals was higher in female patients. The seropositivity to P23 was higher in HIV-infected individuals with high viral load, although the difference was statistically insignificant. Significantly higher Cryptosporidium seropositivity was observed in HIV-infected individuals with a CD4(+) T-cell count of <200 cells/μl than in those with ≥200 cells/μl. Our results also demonstrate that a lower CD4(+) T-cell count may reflect an increased accumulated risk for cryptosporidiosis. The detection system was further validated using the standard ELISA method and good correlation between the two methods was found (r = 0.80). Under the same sensitivity, this new microfluidic chip device had a specificity of 98.2%. This developed system may provide a powerful platform for the fast screening of Cryptospordium infection in HIV-infected patients.
隐孢子虫病已被报道与人类免疫缺陷病毒/获得性免疫缺陷综合征相关，这极大地降低了 HIV 感染患者的生活质量并缩短了其预期寿命。为了对感染患者进行妥善治疗，需要开发准确且自动的诊断工具。在这项研究中，我们提出了一种新型微流控免疫芯片系统，用于监测和快速检测来自中国广西的 190 名 HIV 感染患者的隐孢子虫感染，该系统使用了隐孢子虫的 P23 抗原。该检测程序可在 10 分钟内完成，仅需 2μl 样本量。该系统还使用标准 ELISA 方法进行了评估。在 190 名 HIV 感染个体中，P23 阳性率为 13.7%。女性患者的 HIV 感染个体的血清阳性率更高。尽管差异无统计学意义，但高病毒载量的 HIV 感染个体的 P23 血清阳性率更高。与 CD4(+)T 细胞计数≥200 个/μl 的 HIV 感染个体相比，CD4(+)T 细胞计数<200 个/μl 的 HIV 感染个体的隐孢子虫血清阳性率显著更高。我们的结果还表明，较低的 CD4(+)T 细胞计数可能反映了隐孢子虫病的累积风险增加。该检测系统还使用标准 ELISA 方法进行了验证，两种方法之间存在良好的相关性（r=0.80）。在相同的灵敏度下，这种新的微流控芯片设备具有 98.2%的特异性。该开发系统可为快速筛查 HIV 感染患者的隐孢子虫感染提供强大的平台。
