Birant Sinem, İlisulu Sabiha Ceren, Ozcan Hazal, Yanar Karolin
Faculty of Dentistry, Department of Pediatric Dentistry, Istanbul University-Cerrahpaşa, Cerrahpaşa Yerleşkesi Kocamustafapaşa Caddesi No:53, Cerrahpaşa, Fatih, Istanbul, 34098, Turkey.
Faculty of Dentistry, Department of Pediatric Dentistry, Altinbas University, Istanbul, Turkey.
BMC Oral Health. 2024 Dec 21;24(1):1536. doi: 10.1186/s12903-024-05185-7.
Saliva contains a variety of biochemical compounds, including antioxidants, and serves as the body's first line of defense against oxidative stress caused by free radicals. The aim of this study was to investigate the effects of dental treatments on salivary oxidative stress biomarkers in children aged 3-5 years with severe early childhood caries (S-ECC) compared to children without caries.
This study was conducted on 20 children aged 3-5 years with severe early childhood caries (S-ECC) and 20 children without caries. Salivary oxidative stress biomarkers and antioxidants were measured after the initial examination (T0), after the end of restorative treatments (T1), and after fluoride varnish applications (T2). Post hoc Bonferroni test was used to compare normally distributed parameters between T0-T1-T2 times. Pearson correlation analysis was used to examine the relationships between parameters that conform to normal distribution. The Mann-Whitney U test was used to compare the parameters in the control and experimental groups. Significance was evaluated at the p < 0.05 level.
The mean dmft of the participants in the study group was 8.86 ± 14.5. Advanced oxidation protein products (AOPP), dityrosine (DT), kynurenine (KYN), advanced glycation end products (AGE), lipid hydroperoxides (LHP) and malondialdehyde (MDA) values decrease after the treatment of dental caries and protective fluoride varnish applications, while an increases in total thiol (TSH) and Cu/Zn-superoxide dismutase (Cu/Zn-SOD) values were observed after protective varnish applications compared to pre-treatment values. Antioxidant parameters at time T2 in the study group were statistically significantly higher than in the control group (p < 0.05). In the study group, there was no correlation between TSH and oxidative stress mediators in terms of changes at time T1 post-treatment compared to the pre-treatment period, while an inverse moderate relationship was found with AGE and LHP in terms of changes at time T2 post-treatment (p < 0.05).
An increase in salivary antioxidants was detected after dental restorations were completed and protective fluoride varnish application, while a decrease in oxidative stress markers was detected.
Fluoride varnish applications applied in the study group may further reduce the oral microbiome load and cause salivary oxidative stress markers to be significantly lower than in the control group.
唾液含有多种生化化合物，包括抗氧化剂，是人体抵御自由基引起的氧化应激的第一道防线。本研究的目的是调查与无龋儿童相比，牙科治疗对3至5岁重度幼儿龋齿（S-ECC）儿童唾液氧化应激生物标志物的影响。
本研究对20名3至5岁重度幼儿龋齿（S-ECC）儿童和20名无龋儿童进行。在初次检查后（T0）、修复治疗结束后（T1）和涂氟漆后（T2）测量唾液氧化应激生物标志物和抗氧化剂。采用事后Bonferroni检验比较T0-T1-T2各时间点的正态分布参数。采用Pearson相关分析检验符合正态分布的参数之间的关系。采用Mann-Whitney U检验比较对照组和实验组的参数。以p < 0.05水平评估显著性。
研究组参与者的平均乳牙龋失补牙数（dmft）为8.86 ± 14.5。龋齿治疗和涂用保护性氟漆后，晚期氧化蛋白产物（AOPP）、二酪氨酸（DT）、犬尿氨酸（KYN）、晚期糖基化终产物（AGE）、脂质氢过氧化物（LHP）和丙二醛（MDA）值降低，而与治疗前相比，涂用保护性氟漆后总巯基（TSH）和铜/锌超氧化物歧化酶（Cu/Zn-SOD）值升高。研究组T2时的抗氧化参数在统计学上显著高于对照组（p < 0.05）。在研究组中，与治疗前相比，治疗后T1时TSH与氧化应激介质之间在变化方面无相关性，而在治疗后T2时与AGE和LHP呈中度负相关（p < 0.05）。
完成牙齿修复和涂用保护性氟漆后，检测到唾液抗氧化剂增加，同时氧化应激标志物减少。
研究组涂用的氟漆可能进一步降低口腔微生物负荷，并使唾液氧化应激标志物显著低于对照组。
