Department of Periodontology, Faculty of Dentistry, Ataturk University, Erzurum, Turkey.
Int J Implant Dent. 2024 Oct 14;10(1):43. doi: 10.1186/s40729-024-00562-x.
8-hydroxydeoxyguanosine (8-OHdG) and Malondialdehyde (MDA) are commonly used as markers to evaluate oxidative DNA and Lipid damage in disorders including chronic inflammatory diseases. Superoxide dismutase (SOD) and glutathione peroxidase (GPx) protect tissues against oxidative injury from free oxygen radicals generated by various metabolic processes. The aim of this study was to evaluate 8-OHdG and MDA levels, and SOD and GPx activities in whole saliva of patients with peri-implant diseases.
A cross-sectional study was conducted on a sum of 60 age gender balanced; peri-implantitis (n = 20), peri-mucositis (n = 20) and healthy (n = 20) individuals. Unstimulated whole saliva samples were collected and to determine the clinical condition of each subject; the plaque index (PI), gingival index (GI), peri-implant probing pocket depth (PIPD), peri-implant presence of bleeding on probing (BOP) (with/without suppuration) and radiographic signs of crestal bone loss (BL) were measured. The salivary 8-OHdG level was measured using the ELISA method. SOD, GPx activities and MDA levels were determined spectrophotometrically.
A total of 60 individuals had evaluations of 318 implants. In comparison to the peri-mucositis and peri-implantitis groups, the healthy group had significantly lower PI and GI scores (p < 0.001). The PIPD value differed amongst the groups, with the peri-implantitis group having the highest value (p < 0.001). Compared to the peri-mucositis and control groups, the peri-implantitis group had a significantly higher BL score (p < 0.001 and p < 0.001, respectively). The peri-implantitis group showed a significantly higher 8-OHdG level (p < 0.001; p < 0.001 respectively) than the peri-mucositis and control groups. Compared to the peri-mucositis and control groups, the peri-implantitis group had a significantly higher MDA level (p < 0.001 and p < 0.001, respectively). The peri-implantitis group had a significantly higher SOD level (p < 0.001 and p < 0.001, respectively) in comparison to the peri-mucositis and control groups. There was no significant difference in GPx levels between the peri-mucositis and control groups (p > 0.05), while the peri-implantitis group had significantly lower GPx levels than the peri-mucositis and control groups (p < 0.001 and p < 0.001, respectively).
Elevated levels of oxidative stress in saliva may indicate the onset of pathological bone loss surrounding the implant and may be an indication of peri-implantitis.
In peri-implant diseases, changes may occur in the levels of 8-OHdG, MDA, SOD and GPx in saliva, which may lead to a deterioration in the oxidant/antioxidant balance.
8-羟基脱氧鸟苷（8-OHdG）和丙二醛（MDA）通常被用作评估包括慢性炎症性疾病在内的各种疾病中氧化 DNA 和脂质损伤的标志物。超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）可保护组织免受各种代谢过程中产生的自由基引起的氧化损伤。本研究旨在评估牙周炎、牙周黏膜炎和健康患者全唾液中的 8-OHdG 和 MDA 水平以及 SOD 和 GPx 活性。
对 60 名年龄和性别均衡的牙周炎（n=20）、牙周黏膜炎（n=20）和健康（n=20）患者进行了横断面研究。收集未刺激的全唾液样本，并确定每位受试者的临床状况；菌斑指数（PI）、牙龈指数（GI）、种植体周探诊深度（PIPD）、种植体周探诊出血（BOP）（伴/不伴化脓）和影像学骨嵴丧失（BL）的迹象。采用 ELISA 法测定唾液 8-OHdG 水平。通过分光光度法测定 SOD、GPx 活性和 MDA 水平。
共有 60 名个体对 318 个种植体进行了评估。与牙周黏膜炎和牙周炎组相比，健康组的 PI 和 GI 评分明显较低（p<0.001）。各组间 PIPD 值存在差异，牙周炎组最高（p<0.001）。与牙周黏膜炎和对照组相比，牙周炎组的 BL 评分明显更高（p<0.001 和 p<0.001）。牙周炎组的 8-OHdG 水平明显高于牙周黏膜炎组和对照组（p<0.001；p<0.001）。与牙周黏膜炎和对照组相比，牙周炎组的 MDA 水平明显更高（p<0.001 和 p<0.001）。与牙周黏膜炎和对照组相比，牙周炎组的 SOD 水平明显更高（p<0.001 和 p<0.001）。牙周黏膜炎组和对照组的 GPx 水平无显著差异（p>0.05），而牙周炎组的 GPx 水平明显低于牙周黏膜炎组和对照组（p<0.001 和 p<0.001）。
唾液中氧化应激水平升高可能表明种植体周围发生了病理性骨丧失，并可能是牙周炎的征兆。
在牙周炎疾病中，唾液中 8-OHdG、MDA、SOD 和 GPx 的水平可能会发生变化，这可能导致氧化/抗氧化平衡恶化。
