对表达小鼠胰岛素I启动子-萤火虫荧光素酶转基因的活体小鼠胰腺β细胞进行光学成像。
Optical imaging of pancreatic beta cells in living mice expressing a mouse insulin I promoter-firefly luciferase transgene.
作者信息
Park Soo-Young, Wang Xiaoyu, Chen Zhongyi, Powers Alvin C, Magnuson Mark A, Head W Steven, Piston David W, Bell Graeme I
机构信息
Department of Biochemistry and Molecular Biology, and the Howard Hughes Medical Institute, The University of Chicago, Chicago , Illinois, USA.
出版信息
Genesis. 2005 Oct;43(2):80-6. doi: 10.1002/gene.20157.
We generated transgenic mice expressing firefly (Photinus pyralis) luciferase (luc) under the control of the mouse insulin I promoter (MIP). The mice have normal glucose tolerance and pancreatic islet architecture. The luciferase-expressing beta cells can be readily visualized in living mice using whole-body bioluminescent imaging. The MIP-luc transgenic mice may be useful for monitoring changes in beta cell function or mass in living animals with normal or altered metabolic states.
我们构建了在小鼠胰岛素 I 启动子（MIP）控制下表达萤火虫（Photinus pyralis）荧光素酶（luc）的转基因小鼠。这些小鼠具有正常的糖耐量和胰岛结构。利用全身生物发光成像技术，可在活体小鼠中轻松观察到表达荧光素酶的β细胞。MIP-luc转基因小鼠可能有助于监测处于正常或代谢状态改变的活体动物中β细胞功能或数量的变化。
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