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茉莉酸甲酯和水杨酸对肉苁蓉悬浮培养物中苯乙醇苷合成的影响

[Effects of methyl jasmonate and salicylic acid on phenylethanoid glycosides synthesis in suspension cultures of Cistanche deserticola].

作者信息

Xu Liang-Sheng, Xue Xiao-Feng, Fu Chun-Xiang, Jin Zhi-Ping, Chen Yu-Quan, Zhao De-Xiu

机构信息

Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.

出版信息

Sheng Wu Gong Cheng Xue Bao. 2005 May;21(3):402-6.

Abstract

The present study investigated the influence of the methyl jasmonate and salicylic acid elicitors on the formation of phenylethanoid glycosides (PeG) in the suspension cultures of Cistanche deserticola. The results showed that methyl jasmonate and salicylic acid enhanced greatly the accumulation of PeG and echinacoside (Echin), but their optimum elicitation dosage and addition time were different. The yields of PeG and Echin were significantly increased in the presence of 5 micromol/L methyl jasmonate on day 14 (up to 2.59-fold and 3.82-fold, respectively), whereas treated with 50 micromol/L salicylic acid on day 28, the maximum content of them were, respectively, 2.71 and 3.16-fold higher than the untreated cell cultures.

摘要

本研究考察了茉莉酸甲酯和水杨酸诱导剂对肉苁蓉悬浮培养物中苯乙醇苷(PeG)形成的影响。结果表明,茉莉酸甲酯和水杨酸极大地促进了PeG和松果菊苷(Echin)的积累,但它们的最佳诱导剂量和添加时间不同。在第14天添加5 μmol/L茉莉酸甲酯时,PeG和Echin的产量显著增加(分别高达2.59倍和3.82倍),而在第28天用50 μmol/L水杨酸处理时,它们的最大含量分别比未处理的细胞培养物高2.71倍和3.16倍。

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