肉苁蓉愈伤组织在固体培养基上生长形成苯乙醇苷类化合物。
Formation of phenylethanoid glycosides by Cistanche deserticola callus grown on solid media.
作者信息
Ouyang Jie, Wang Xiao-dong, Zhao Bing, Wang Yu-chun
机构信息
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, PO Box 353, Beijing 100080, China.
出版信息
Biotechnol Lett. 2003 Feb;25(3):223-5. doi: 10.1023/a:1022325203622.
The optimal growth of Cistanche deserticola callus and formation of phenylethanoid glycosides (PeG) was at 25 degrees C with light irradiation intensity of 24 micromol m(-2) s(-1) on solidified B5 media supplemented with 0.5 mg 6-benzylaminopurine l(-1), 10 mg gibberellin l(-1), 800 mg casein hydrolysate l(-1) and 20 g sucrose l(-1). After 30 d culture, the biomass reached 15.5 g dry wt callus l(-1) medium and its PEG content was 10.7% (w/w). The PeG content was 42%-127% higher than those in explants.
肉苁蓉愈伤组织的最佳生长及苯乙醇苷（PeG）的形成条件为：在添加了0.5 mg/L 6-苄基腺嘌呤、10 mg/L赤霉素、800 mg/L水解酪蛋白和20 g/L蔗糖的固化B5培养基上，温度25℃，光照强度24 μmol m⁻² s⁻¹。培养30 d后，生物量达到15.5 g干重愈伤组织/L培养基，其PEG含量为10.7%（w/w）。PeG含量比外植体中的高42%-127%。
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