壳聚糖诱导剂对肉苁蓉细胞悬浮培养物中苯乙醇苷生物合成的促进作用

Improvement of phenylethanoid glycosides biosynthesis in Cistanche deserticola cell suspension cultures by chitosan elicitor.

作者信息

Cheng Xi-Yu, Zhou Hua-Ying, Cui Xin, Ni Wen, Liu Chun-Zhao

机构信息

State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, PR China.

出版信息

J Biotechnol. 2006 Jan 24;121(2):253-60. doi: 10.1016/j.jbiotec.2005.07.012. Epub 2005 Sep 6.

DOI:10.1016/j.jbiotec.2005.07.012

PMID:16143416

Abstract

Effect of chitosan elicitor on growth and phenylethanoid glycosides (PeGs) accumulation in Cistanche deserticola cell suspension cultures was investigated. PeGs accumulation was dramatically improved by addition of selected chitosan at optimal elicitation conditions. Furthermore, a strategy of repeated addition of the chitosan elicitor for enhancing PeGs accumulation was developed. The chitosan elicitor of 10 mg l(-1)-medium repeatedly added on days 15 and 17 improved PeGs accumulation further, and the final PeGs production in the treated cell cultures of C. deserticola reached 364.6 mg l(-1), which was 3.4-fold higher than that of the control without elicitation. The increase of PeGs accumulation in C. deserticola cell suspension cultures was related to the increase of phenylalanine ammonium lyase activity stimulated by the chitosan elicitor.

摘要

研究了壳聚糖激发子对肉苁蓉细胞悬浮培养物生长及苯乙醇苷（PeGs）积累的影响。在最佳诱导条件下添加选定的壳聚糖可显著提高PeGs的积累。此外，还开发了一种通过重复添加壳聚糖激发子来增强PeGs积累的策略。在第15天和第17天重复添加10 mg l(-1)培养基的壳聚糖激发子进一步提高了PeGs的积累，处理后的肉苁蓉细胞培养物中PeGs的最终产量达到364.6 mg l(-1)，比未诱导的对照高出3.4倍。肉苁蓉细胞悬浮培养物中PeGs积累的增加与壳聚糖激发子刺激苯丙氨酸解氨酶活性的增加有关。