Niessen J, Schröder U, Harnisch F, Scholz F
Institut für Chemie und Biochemie, Universität Greifswald, Greifswald, Germany.
Lett Appl Microbiol. 2005;41(3):286-90. doi: 10.1111/j.1472-765X.2005.01742.x.
To exploit the fermentative hydrogen generation and direct hydrogen oxidation for the generation of electric current from the degradation of cellulose.
Utilizing the metabolic activity of the mesophilic anaerobe Clostridium cellulolyticum and the thermophilic Clostridium thermocellum we show that electricity generation is possible from cellulose fermentation. The current generation is based on an in situ oxidation of microbially synthesized hydrogen at platinum-poly(tetrafluoroaniline) (Pt-PTFA) composite electrodes. Current densities of 130 mA l(-1) (with 3 g cellulose per litre medium) were achieved in poised potential experiments under batch and semi-batch conditions.
The presented results show that electricity generation is possible by the in situ oxidation of hydrogen, product of the anaerobic degradation of cellulose by cellulolytic bacteria.
For the first time, it is shown that an insoluble complex carbohydrate like cellulose can be used for electricity generation in a microbial fuel cell. The concept represents a first step to the utilization of macromolecular biomass components for microbial electricity generation.
利用纤维素降解过程中的发酵产氢和直接氢氧化作用来产生电流。
利用嗜温厌氧菌解纤维素梭菌和嗜热嗜热栖热菌的代谢活性，我们证明了通过纤维素发酵可以发电。电流产生基于在铂 - 聚（四氟苯胺）（Pt - PTFA）复合电极上对微生物合成氢的原位氧化。在分批和半分批条件下的恒电位实验中，每升培养基含3克纤维素时实现了130 mA l(-1)的电流密度。
所呈现的结果表明，通过纤维素分解菌对纤维素进行厌氧降解产生的氢的原位氧化可以发电。
首次表明像纤维素这样的不溶性复合碳水化合物可用于微生物燃料电池发电。这一概念代表了利用大分子生物质成分进行微生物发电的第一步。
