Väisänen Timo, Väisänen Marja-Riitta, Autio-Harmainen Helena, Pihlajaniemi Taina
Collagen Research Unit, Biocenter Oulu and Department of Medical Biochemistry and Molecular Biology, University of Oulu, PO Box 5000, 90014 University of Oulu, Finland.
J Pathol. 2005 Nov;207(3):324-35. doi: 10.1002/path.1836.
Little information is available on the expression of transmembrane type XIII collagen in human diseases. The present study has investigated the expression of this collagen in cancer, in particular during malignant transformation. By combining the tissue microarray technique with in situ hybridization, a consistent pattern of clearly increased type XIII collagen mRNA expression was found in the stromal compartment of epithelial tumours and throughout mesenchymal tumours. Slightly elevated mRNA expression was observed in dysplastic samples and in malignant epithelial cells. It is also demonstrated that factors secreted into the culture medium by tumour cells, in particular the growth factor TGF-beta, contribute to the induction of type XIII collagen expression, and trigger concomitantly a profound phenotypic and morphological transition of cultured primary fibroblasts. Reciprocally, type XIII collagen may alter the growth milieu of malignant cells as the soluble type XIII collagen ectodomain influenced the adherence and spreading of cells cultured on vitronectin-rich matrix. It is proposed that malignant transformation stimulates the expression of type XIII collagen, particularly in the tumour stroma and to a lesser extent in the epithelium, and that this high type XIII collagen expression may contribute to tumour progression and behaviour by modulating cell-matrix interactions.
关于跨膜型 XIII 胶原蛋白在人类疾病中的表达情况，目前所知甚少。本研究调查了这种胶原蛋白在癌症中的表达，特别是在恶性转化过程中的表达。通过将组织微阵列技术与原位杂交相结合，发现在上皮肿瘤的基质区以及整个间充质肿瘤中，XIII 型胶原蛋白 mRNA 表达呈现出一致且明显增加的模式。在发育异常的样本和恶性上皮细胞中观察到 mRNA 表达略有升高。研究还表明，肿瘤细胞分泌到培养基中的因子，特别是生长因子 TGF-β，有助于诱导 XIII 型胶原蛋白的表达，并同时引发培养的原代成纤维细胞深刻的表型和形态转变。相反，XIII 型胶原蛋白可能会改变恶性细胞的生长环境，因为可溶性 XIII 型胶原蛋白胞外域会影响在富含玻连蛋白的基质上培养的细胞的黏附和铺展。有人提出，恶性转化会刺激 XIII 型胶原蛋白的表达，特别是在肿瘤基质中，在上皮中程度较轻，并且这种高水平的 XIII 型胶原蛋白表达可能通过调节细胞与基质的相互作用而促进肿瘤进展和行为。
