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Development of a real-time probe-based PCR assay for the diagnosis of Pneumocystis pneumonia.

作者信息

Strutt Matthew, Smith Melvyn

机构信息

Health Protection Agency London, Guy's, Kings and St Thomas' School of Medicine, Kings College Hospital, London, UK.

出版信息

Med Mycol. 2005 Jun;43(4):343-7. doi: 10.1080/13693780412331282340.

DOI:10.1080/13693780412331282340
PMID:16110780
Abstract

This paper describes the development of a rapid probe-based real-time polymerase chain reaction assay (PCR) for the diagnosis of Pneumocystis pneumonia. To develop the PCR, primers and fluorescent resonance energy transfer probes were designed after sequencing products obtained using previously published primers. This gave results that were concordant with conventional cytological staining techniques, but were available within 2 h instead of greater than 24 h.

摘要

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