[苦苣菜的组织培养与植株再生]
[Tissue culture and plant regeneration of Ixeridium sonchifolium].
作者信息
Tao Jing, Yang Shi-Hai, Zhang Meng-Ping, Tian Yi-Xin
机构信息
College of Chinese Medicinal Material, Jilin Agricultural University, Changchun 130118, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2008 Feb;33(4):368-71.
OBJECTIVE
To study the callus induction and plant regeneration of Ixeridium sonchifolium.
METHOD
By using the orthogonal experiment design, the medium for callus induction and plant regeneration was optimized.
RESULT
The optimal medium for callus induction was MS + 2, 4-D 1.5 mg x L(-1) +6-BA 1.5 mg x L(-1) +NAA 1.0 mg x L(-1) + IBA 1.5 mg x L(-1) + KT1.5 mg x L(-1), the optimal medium for inducing adventitious bud was MS +2, 4-D 0.2 mg x L(-1) +6-BA 0.5 mg x L(-1) + NAA 0.5 mg x L(-1) + IBA 0.5 mg x L(-1) + KT 0.5 mg x L(-1). Plantlets were rooted on 1/4MS medium supplemented with different concentrations of IBA, and high rooting and survival was achieved when the IBA concentration was 0.1 mg L(-1).
CONCLUSION
An efficient system for plant regeneration of I. sonchifolium was established.
目的
研究苦苣菜的愈伤组织诱导及植株再生。
方法
采用正交试验设计，对苦苣菜愈伤组织诱导及植株再生的培养基进行优化。
结果
愈伤组织诱导的最佳培养基为MS + 2,4 - D 1.5 mg·L⁻¹ + 6 - BA 1.5 mg·L⁻¹ + NAA 1.0 mg·L⁻¹ + IBA 1.5 mg·L⁻¹ + KT 1.5 mg·L⁻¹；不定芽诱导的最佳培养基为MS + 2,4 - D 0.2 mg·L⁻¹ + 6 - BA 0.5 mg·L⁻¹ + NAA 0.5 mg·L⁻¹ + IBA 0.5 mg·L⁻¹ + KT 0.5 mg·L⁻¹。在添加不同浓度IBA的1/4MS培养基上进行生根培养，当IBA浓度为0.1 mg·L⁻¹时，生根率和成活率较高。
结论
建立了高效的苦苣菜植株再生体系。
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