Torres-Acosta J F J, Aguilar-Caballero A J, Le Bigot C, Hoste H, Canul-Ku H L, Santos-Ricalde R, Gutiérrez-Segura I
Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Yucatán. Mérida, Yucatán, México. Km 15.5 carretera Mérida-Xmatkuil, Mérida, Yucatán.
Vet Parasitol. 2005 Dec 10;134(3-4):241-8. doi: 10.1016/j.vetpar.2005.07.012. Epub 2005 Aug 18.
The objective was to examine the coincidence in the prevalence of benzimidazole (Bz) resistance in smallholder goat herds, as determined by three average-based and two individually-based faecal egg count reduction (FECR) tests. Nineteen smallholder goat herds with more than 30 animals were selected from 84 herds in Yucatan. Animals shedding 150 eggs/g of faeces (EPG) on day zero were randomly divided into two groups. The control group did not receive treatment and the treated group received fenbendazole (10mg/kg body weight per os). Feed was withdrawn for 16 h before treatment. Ten days after treatment, both groups were sampled to determine their FEC. Faecal cultures and identification of infective larvae were performed for estimating the proportions of genera of gastrointestinal nematodes (GIN) present. Presence of resistant GIN was determined with three different average-based FECR (FECR(1), FECR(2) and FECR(3)) and two individually-based FECR (iFECR(1) and iFECR(2)). The prevalence of herds with Bz resistant nematodes (and 95% confidence interval (95% CI)) was calculated using each formula. Coincidence among formulae was estimated with Kappa values. The prevalence (+/- 95% CI) of Bz resistance calculated with FECR(1) (57.89 +/- 22.20) had a high coincidence with iFECR(1) and iFECR(2) (Kappa values of 0.86 and 0.79, respectively). The prevalence with FECR(2) (31.58 +/- 20.90) and FECR(3) (21.05 +/- 18.33) had a low coincidence with FECR(1) (Kappa < 0.50). Trichostrongylids found on Bz resistant farms were mainly Haemonchus spp., however, some Trichostrongylus spp. and Oesophagostomum spp. were found too. The high coincidence between the standard average-based FECR(1) and the individually based formulae is encouraging and may suggest that either formula could be applied to smallholder farmers. Further laboratory studies are needed to confirm the resistance status in the herds.
目的是通过三种基于平均值的和两种基于个体的粪便虫卵计数减少（FECR）试验，检测小农户山羊群中苯并咪唑（Bz）抗性的流行情况是否一致。从尤卡坦半岛的84个牧群中挑选出19个存栏超过30只动物的小农户山羊群。在第零天粪便虫卵数为150个/克（EPG）的动物被随机分为两组。对照组不接受治疗，治疗组口服芬苯达唑（10毫克/千克体重）。治疗前禁食16小时。治疗10天后，对两组进行采样以确定其粪便虫卵计数。进行粪便培养和感染性幼虫鉴定，以估计存在的胃肠线虫（GIN）属的比例。使用三种不同的基于平均值的FECR（FECR(1)、FECR(2)和FECR(3)）以及两种基于个体的FECR（iFECR(1)和iFECR(2)）来确定抗性GIN的存在情况。使用每个公式计算具有Bz抗性线虫的牧群的流行率（及95%置信区间（95%CI））。用Kappa值估计公式之间的一致性。用FECR(1)计算的Bz抗性流行率（57.89 +/- 22.20）与iFECR(1)和iFECR(2)具有高度一致性（Kappa值分别为0.86和0.79）。FECR(2)（31.58 +/- 20.90）和FECR(3)（21.05 +/- 18.33）的流行率与FECR(1)的一致性较低（Kappa < 0.50）。在有Bz抗性的农场中发现的毛圆科线虫主要是血矛线虫属，不过也发现了一些毛圆线虫属和食道口线虫属。基于标准平均值的FECR(1)与基于个体的公式之间的高度一致性令人鼓舞，这可能表明任何一种公式都可应用于小农户。需要进一步的实验室研究来确认这些牧群中的抗性状况。
