Mickiewicz Marcin, Czopowicz Michał, Górski Paweł, Kaba Jarosław
Laboratory of Veterinary Epidemiology and Economics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, ul. Nowoursynowska 159c, 02-776 Warsaw, Poland
Division of Parasitology and Invasiology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, ul. Ciszewskiego 8, 02-787 Warsaw, Poland
Ann Parasitol. 2017;63(4):317-322. doi: 10.17420/ap6304.118.
Fecal egg count reduction (FECR) test with albendazole and egg hatch test (EHT) with thiabendazole (TBZ) were performed in a dairy goat herd suspected of anthelmintic resistance to benzimidazoles. The herd had been regularly dewormed with fenbendazole for 5 previous years and despite that it remained infected with several species of gastrointestinal nematodes (Trichostrongylus colubriformis, Teladorsagia circumcincta, and Haemonchus contortus). Albendazole was administered per os at dose of 20 mg/kg to 10 goats (treated group), while 10 other goats remained untreated (control group). Fecal egg count (FEC) was determined using McMaster egg counting method before and 7 days after the treatment in the treated group, and once (at the latter moment) in the control group. EHT was performed on the pooled rectal sample collected from treated goats. EHT comprised the negative control and 7 consecutive concentrations of TBZ (0.05, 0.1, 0.2, 0.3, 0.5, 1.0, 2.0 μg/ml) according to the standard procedure. Two hundred eggs/larvae were counted to determine percentage of unhatched eggs, which was adjusted by the natural mortality. TBZ dose effective in preventing hatching of 50% of eggs (ED50) was determined using the log-probit transformation. Median FEC (range) before the treatment was 1000 (250–3450) epg in the treated group and dropped to 150 (50–500) epg after the treatment (p=0.005). Median FEC (range) after the treatment was also significantly lower in the treated than in control group (p=0.009), where it was 725 (0–5050) epg. FECR between the treated and control group was 81% (95% CI: 49%, 93%). FECR in the treated group was 83% and 74% based on average and individual approach, respectively. ED50 value of TBZ was 0.78 μg/ml. Only H. contortus persisted in the treated group after treatment. The results indicate resistance of H. contortus to a benzimidazole anthelmintic, which is the first such case reported in Polish goats.
在一个怀疑对苯并咪唑类驱虫药产生抗药性的奶山羊群中，进行了阿苯达唑粪便虫卵计数减少（FECR）试验和噻苯达唑（TBZ）虫卵孵化试验（EHT）。该羊群在过去5年中一直定期用芬苯达唑驱虫，但尽管如此，它仍感染了几种胃肠道线虫（蛇形毛圆线虫、环形泰勒虫和捻转血矛线虫）。给10只山羊（治疗组）口服20mg/kg剂量的阿苯达唑，而另外10只山羊不进行治疗（对照组）。在治疗组治疗前和治疗后7天使用麦克马斯特虫卵计数法测定粪便虫卵计数（FEC），在对照组只在治疗后7天测定一次（后者时间点）。对从治疗山羊收集的混合直肠样本进行EHT。EHT按照标准程序包括阴性对照和7个连续浓度的TBZ（0.05、0.1、0.2、0.3、0.5、1.0、2.0μg/ml）。计数200个虫卵/幼虫以确定未孵化虫卵的百分比，并根据自然死亡率进行调整。使用对数概率转换法确定能防止50%虫卵孵化的TBZ剂量（ED50）。治疗组治疗前的FEC中位数（范围）为1000（250 - 3450）个虫卵/克，治疗后降至150（50 - 500）个虫卵/克（p = 0.005）。治疗组治疗后的FEC中位数（范围）也显著低于对照组（p = 0.009），对照组为725（0 - 5050）个虫卵/克。治疗组与对照组之间的FECR为81%（95%CI：49%，93%）。基于平均和个体方法，治疗组的FECR分别为83%和74%。TBZ的ED50值为0.78μg/ml。治疗后治疗组中仅捻转血矛线虫持续存在。结果表明捻转血矛线虫对苯并咪唑类驱虫药产生了抗药性，这是波兰山羊中首次报道的此类病例。
