Muhlia-Almazán Adriana, Sánchez-Paz Arturo, García-Carreño Fernando, Peregrino-Uriarte Alma Beatriz, Yepiz-Plascencia Gloria
Aquatic Molecular Biology, Centro de Investigación en Alimentación y Desarrollo, A.C., P.O. Box 1735, Hermosillo, Sonora 83000, México.
Comp Biochem Physiol B Biochem Mol Biol. 2005 Oct;142(2):209-16. doi: 10.1016/j.cbpc.2005.07.005.
A high density lipoprotein-beta glucan binding protein (HDL-BGBP) is synthesized in the hepatopancreas of the white shrimp Litopenaeus vannamei and secreted to the hemolymph. Recently, we reported the HDL-BGBP full length cDNA sequence and found that the predicted polypeptide is larger than the mature protein and also, that it contains a long 5'- and 3'-UTRs that may be involved in transcript level regulation. To test whether starvation and feeding may play a role in regulating HDL-BGBP mRNA levels, two different stimuli were evaluated: starvation and composition of diets. After 24 h, the steady state HDL-BGBP mRNA levels of starved shrimp decreased, suggesting that synthesis of the lipoprotein is less required in the absence of food. When shrimp were fed with diets containing different concentrations of protein and lipids, changes in HDL-BGBP mRNA levels were also detected. Shrimp fed the lower concentration of protein and lipid feed accumulated higher levels of HDL-BGBP mRNA. These results indicate that feeding influences HDL-BGBP transcript levels in the hepatopancreas.
一种高密度脂蛋白-β-葡聚糖结合蛋白（HDL-BGBP）在凡纳滨对虾的肝胰腺中合成并分泌到血淋巴中。最近，我们报道了HDL-BGBP的全长cDNA序列，发现预测的多肽比成熟蛋白大，而且它含有可能参与转录水平调控的长5'-和3'-非翻译区。为了测试饥饿和投喂是否可能在调节HDL-BGBP mRNA水平中起作用，评估了两种不同的刺激：饥饿和饲料组成。24小时后，饥饿对虾的HDL-BGBP mRNA稳态水平下降，这表明在没有食物的情况下对脂蛋白合成的需求较少。当对虾投喂含有不同浓度蛋白质和脂质的饲料时，也检测到了HDL-BGBP mRNA水平的变化。投喂低浓度蛋白质和脂质饲料的对虾积累了更高水平的HDL-BGBP mRNA。这些结果表明，投喂会影响肝胰腺中HDL-BGBP的转录水平。
