Goldschmith A, Infante C, Leiva J, Motles E, Palestini M
Facultad de Ingeniería, Escuela de Geología, Universidad de Chile, Chile.
Brain Res. 2005 Sep 21;1056(2):176-82. doi: 10.1016/j.brainres.2005.07.030.
The objective of our study was to find the evidence of copper interaction in LTP, motivated by copper involvement in neurodegenerative illness, like Parkinson, Alzheimer and Amyotrophic Lateral Sclerosis, and we initiated the study of this element in the LTP. For this purpose we used hippocampus slices of rats chronically consuming copper dissolved in water (CuDR; n=26) and non-copper-consuming rats (CR; n=20). The CuDR rats received 8--10 mg/day during 20--25 days. Electrophysiological tests showed absence of LTP in CuDR slices, contrary to CR slices. The stimulus-response test applied before and after LTP showed significant increases of synaptic potential in the CR group. This did not occur in the CuDR group, except for the initial values, which probably seem associated to an early action of copper. The paired-pulse (PP) test, applied to CR and CuDR prior to tetanic stimulation, showed a significant reduction in PP, for the 20-, 30- and 50-ms intervals in CuDR. At the end of the experiments, copper concentration was 54.2 times higher in CuDR slices, compared to the concentration present in CR slices. Our results show that copper reduces synaptic sensibility and also the facilitation capability. These effects represent a significant disturbance in the plasticity phenomenon associated with learning and memory.
我们研究的目的是寻找铜在长时程增强（LTP）中相互作用的证据，这是受铜参与神经退行性疾病（如帕金森病、阿尔茨海默病和肌萎缩侧索硬化症）的启发，我们启动了对该元素在LTP中的研究。为此，我们使用了长期饮用溶解在水中的铜的大鼠（CuDR；n = 26）和不饮用铜的大鼠（CR；n = 20）的海马切片。CuDR大鼠在20 - 25天内每天接受8 - 10毫克铜。电生理测试显示，与CR切片相反，CuDR切片中不存在LTP。在LTP前后进行的刺激 - 反应测试显示，CR组的突触电位显著增加。CuDR组除了初始值外没有出现这种情况，初始值可能与铜的早期作用有关。在强直刺激之前对CR和CuDR应用的配对脉冲（PP）测试显示，在CuDR中，20毫秒、30毫秒和50毫秒间隔的PP显著降低。在实验结束时，CuDR切片中的铜浓度比CR切片中的浓度高54.2倍。我们的结果表明，铜会降低突触敏感性以及易化能力。这些影响代表了与学习和记忆相关的可塑性现象的显著干扰。
