利用病毒载体在植物中生产乙肝病毒核心颗粒。
The use of viral vectors to produce hepatitis B virus core particles in plants.
作者信息
Mechtcheriakova I A, Eldarov M A, Nicholson L, Shanks M, Skryabin K G, Lomonossoff G P
机构信息
Laboratory of Genetic Engineering, Centre Bioengineering RAS, Prospekt 60-Letya Oktyabrya, 7/1, 117312 Moscow, Russian Federation.
出版信息
J Virol Methods. 2006 Jan;131(1):10-5. doi: 10.1016/j.jviromet.2005.06.020. Epub 2005 Aug 19.
The expression and assembly of the hepatitis B virus (HBV) nucleocapsid protein (HBcAg) were investigated in plants using viral vectors. Constructs based on either Potato virus X (PVX) or Cowpea mosaic virus (CPMV) containing the sequence of HBcAg were able to infect the appropriate host plants and remained genetically stable during infection. Analysis of HBcAg expression revealed that the protein can self-assemble into core-like particles and that the assembled material could be partially purified by differential centrifugation. Thus, the use of viral vectors can be considered a practical method for rapid production of assembled HBcAg particles in plants. This approach provides a means whereby a variety of chimaeric particles can be assessed quickly and cheaply for various diagnostic and vaccine applications.
利用病毒载体在植物中研究了乙型肝炎病毒（HBV）核衣壳蛋白（HBcAg）的表达与组装。基于马铃薯X病毒（PVX）或豇豆花叶病毒（CPMV）构建的含有HBcAg序列的载体能够感染相应的宿主植物，并且在感染过程中保持遗传稳定。对HBcAg表达的分析表明，该蛋白能够自组装成核心样颗粒，并且组装后的物质可以通过差速离心进行部分纯化。因此，使用病毒载体可被视为在植物中快速生产组装好的HBcAg颗粒的一种实用方法。这种方法提供了一种手段，通过它可以快速且低成本地评估各种嵌合颗粒在各种诊断和疫苗应用中的效果。
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