Chary Parag, Njenga M Kariuki, Sharma Jagdev M
Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA.
Vet Immunol Immunopathol. 2005 Dec 15;108(3-4):427-32. doi: 10.1016/j.vetimm.2005.06.012. Epub 2005 Aug 19.
Protection by recombinant avian metapneumovirus (aMPV) N or M proteins against a respiratory challenge with virulent aMPV was examined. N, M or N+M proteins were administered intramuscularly (IM) with incomplete Freund's adjuvant (IFA) or by the oculonasal (ON) route with cholera toxin-B (CTB). Each turkey received 40 or 80 microg of each recombinant protein. Birds were considered protected against challenge if the challenge virus was not detectable in the choanal swabs by RT-PCR. At a dose of 40 microg/bird, N protein given with IFA by the IM route protected eight out of nine birds. M protein at the same dose protected three out of seven birds, while a combination of N+M proteins (40 microg each) protected three out of four birds. At a dose of 80 microg of each of N and M proteins per bird given with IFA by the IM route, 100% protection was achieved. ON immunization with a mixture of N and M proteins induced partial protection when the proteins were given with CTB; no detectable protection was noted without CTB. N and M proteins induced anti-aMPV antibodies, although protection against virulent virus challenge did not appear to be associated with the level or presence of antibodies.
研究了重组禽偏肺病毒（aMPV）N或M蛋白对强毒aMPV呼吸道攻击的保护作用。N、M或N+M蛋白通过不完全弗氏佐剂（IFA）肌肉注射（IM）或通过霍乱毒素B（CTB）经眼鼻（ON）途径给药。每只火鸡接受40或80微克的每种重组蛋白。如果通过RT-PCR在鼻后孔拭子中未检测到攻击病毒，则认为鸟类受到保护而免受攻击。以每只鸟40微克的剂量，通过IM途径与IFA一起给予的N蛋白保护了9只鸟中的8只。相同剂量的M蛋白保护了7只鸟中的3只，而N+M蛋白组合（各40微克）保护了4只鸟中的3只。以每只鸟80微克的剂量，通过IM途径与IFA一起给予N和M蛋白，可实现100%的保护。当N和M蛋白与CTB一起给予时，通过ON免疫诱导了部分保护；没有CTB时未观察到可检测到的保护。N和M蛋白诱导了抗aMPV抗体，尽管对强毒病毒攻击的保护似乎与抗体水平或存在无关。
