Ribeiro Cecilia C C, Tabchoury Cínthia P M, Del Bel Cury Altair A, Tenuta Livia M A, Rosalen Pedro L, Cury Jaime A
Faculty of Dentistry of Piracicaba, University of Campinas, Av. Limeira 901, CEP 13414-903, Piracicaba, São Paulo, Brazil.
Br J Nutr. 2005 Jul;94(1):44-50. doi: 10.1079/bjn20051452.
Since in vitro and animal studies suggest that the combination of starch with sucrose may be more cariogenic than sucrose alone, the study assessed in situ the effects of this association applied in vitro on the acidogenicity, biochemical and microbiological composition of dental biofilm, as well as on enamel demineralization. During two phases of 14 d each, fifteen volunteers wore palatal appliances containing blocks of human deciduous enamel, which were extra-orally submitted to four groups of treatments: water (negative control, T1); 2 % starch (T2); 10 % sucrose (T3); and 2 % starch+10 % sucrose (T4). The solutions were dripped onto the blocks eight times per day. The biofilm formed on the blocks was analysed with regard to amylase activity, acidogenicity, and biochemical and microbiological composition. Demineralization was determined on enamel by cross-sectional microhardness. The greatest mineral loss was observed for the association starch+sucrose (P<0.05). Also, this association resulted in the highest lactobacillus count in the biofilm formed (P<0.05). In conclusion, the findings suggest that a small amount of added starch increases the cariogenic potential of sucrose.
由于体外和动物研究表明，淀粉与蔗糖的组合可能比单独的蔗糖更具致龋性，该研究在体内评估了这种体外应用的组合对牙菌斑的产酸性、生化和微生物组成以及牙釉质脱矿的影响。在两个为期14天的阶段中，15名志愿者佩戴含有人类乳牙牙釉质块的腭部装置，这些装置在口外接受四组处理：水（阴性对照，T1）；2%淀粉（T2）；10%蔗糖（T3）；以及2%淀粉+10%蔗糖（T4）。溶液每天滴在牙釉质块上8次。对牙釉质块上形成的生物膜进行淀粉酶活性、产酸性以及生化和微生物组成分析。通过横断面显微硬度测定牙釉质的脱矿情况。观察到淀粉+蔗糖组合导致的矿物质损失最大（P<0.05）。此外，这种组合导致形成的生物膜中乳酸杆菌数量最高（P<0.05）。总之，研究结果表明，少量添加的淀粉会增加蔗糖的致龋潜力。
