Duggleby S L, Waterlow J C
MRC Epidemiology Resource Centre, Southampton General Hospital, Southampton 5022 5A, UK.
Br J Nutr. 2005 Aug;94(2):141-53. doi: 10.1079/bjn20051460.
The present review summarizes the results of all published papers on whole-body protein turnover in man measured by [15N]glycine and the end-product method using both urea and ammonia. It begins with a short account of the underlying assumptions and the justification for the use of [15N]glycine. The results are then compared with those of a large sample of measurements by the 'gold standard' precursor method with continuous infusion of [13C]leucine. The pros and cons of the two methods are compared and it is suggested that there is a place for further work by the less invasive end-product method, particularly for population studies of the genetic, environmental and functional determinants of whole-body rates of protein synthesis.
本综述总结了所有已发表的关于用[15N]甘氨酸和使用尿素及氨的终产物法测量人体全身蛋白质周转率的论文结果。首先简要介绍了基本假设以及使用[15N]甘氨酸的理由。然后将这些结果与通过连续输注[13C]亮氨酸的“金标准”前体法进行的大量测量结果进行比较。比较了这两种方法的优缺点，并提出侵入性较小的终产物法还有进一步研究的空间，特别是在对全身蛋白质合成速率的遗传、环境和功能决定因素进行人群研究方面。
