Thakkar A P, Dhamankar V S, Kapadnis B P
Department of Microbiology, University of Pune, Pune 411 007, India.
Bioresour Technol. 2006 Aug;97(12):1377-81. doi: 10.1016/j.biortech.2005.07.005. Epub 2005 Aug 22.
Bioremediation potential of Phanerochaete chrysosporium strains NCIM 1073, NCIM 1106 and NCIM 1197 to decolourise molasses in solid and liquid molasses media was studied. Strains varied in the pattern of molasses decolourisation on solid medium by Giant colony method. Under submerged cultivation conditions, strain NCIM 1073 did not decolourise molasses while, strains NCIM 1106 and NCIM 1197 could decolourise molasses up to 82% and 76%, respectively. Under stationary cultivation conditions, none of the strains could decolourise molasses. This was overcome by increasing the surface area of the culture in flat bottom glass bottles under stationary cultivation conditions. Under submerged cultivation conditions, growth was more or less same in all strains. However, the lignin peroxidase and manganese peroxidase activities were significantly less in the strain NCIM 1073. Under stationary cultivation conditions, none of the strains could produce enzymes lignin peroxidase, manganese peroxidase and laccase. However, all of them could produce lignin peroxidase and manganese peroxidase when cultivated in flat bottom glass bottles under stationary cultivation conditions.
研究了黄孢原毛平革菌菌株NCIM 1073、NCIM 1106和NCIM 1197在固体和液体糖蜜培养基中对糖蜜进行脱色的生物修复潜力。通过巨型菌落法，各菌株在固体培养基上的糖蜜脱色模式有所不同。在深层培养条件下，菌株NCIM 1073不能使糖蜜脱色，而菌株NCIM 1106和NCIM 1197分别能使糖蜜脱色达82%和76%。在静置培养条件下，所有菌株均不能使糖蜜脱色。在静置培养条件下，通过增加平底玻璃瓶中培养物的表面积克服了这一问题。在深层培养条件下，所有菌株的生长情况大致相同。然而，菌株NCIM 1073中的木质素过氧化物酶和锰过氧化物酶活性明显较低。在静置培养条件下，所有菌株均不能产生木质素过氧化物酶、锰过氧化物酶和漆酶。然而，当在静置培养条件下于平底玻璃瓶中培养时，它们均能产生木质素过氧化物酶和锰过氧化物酶。
