College of Environmental Science and Engineering, Hunan University, Changsha 410082, People's Republic of China.
Appl Microbiol Biotechnol. 2011 Aug;91(3):811-21. doi: 10.1007/s00253-011-3313-4. Epub 2011 May 10.
Phanerochaete chrysosporium has been recognised as an effective bioremediation agent due to its unique degradation to xenobiotic and biosorption ability to heavy metals. However, few studies have focused on the simultaneous removal of heavy metals and organic pollutants. The aim of this work was to study the feasibility of simultaneous cadmium removal and 2,4-dichlorophenol (2,4-DCP) degradation in P. chrysosporium liquid cultures. The removal efficiencies were pH dependent and the maximum removal efficiencies were observed at pH 6.5 under an initial cadmium concentration of 5 mg/L and an initial 2,4-DCP concentration of 20 mg/L. The removal efficiencies for cadmium and 2,4-DCP reached 63.62% and 83.90%, respectively, under the optimum conditions. The high production levels of lignin peroxidase (7.35 U/mL) and manganese peroxidase (8.30 U/mL) resulted in an increase in 2,4-DCP degradation. The protein content decreased with increasing cadmium concentration. The surface characteristics and functional groups of the biomass were studied by scanning electron microscopy and a Fourier-transformed infrared spectrometer. The results showed that the use of P. chrysosporium is promising for the simultaneous removal of cadmium and 2,4-DCP from liquid media.
黄孢原毛平革菌由于其对异生物质的独特降解能力和对重金属的生物吸附能力，已被公认为一种有效的生物修复剂。然而，很少有研究关注同时去除重金属和有机污染物。本工作旨在研究黄孢原毛平革菌液体培养物中同时去除镉和 2,4-二氯苯酚（2,4-DCP）的可行性。去除效率与 pH 值有关，在初始镉浓度为 5mg/L 和初始 2,4-DCP 浓度为 20mg/L 时，在 pH 值为 6.5 时观察到最大去除效率。在最佳条件下，镉和 2,4-DCP 的去除效率分别达到 63.62%和 83.90%。木质素过氧化物酶（7.35 U/mL）和锰过氧化物酶（8.30 U/mL）的高产量导致 2,4-DCP 降解增加。随着镉浓度的增加，蛋白质含量下降。通过扫描电子显微镜和傅里叶变换红外光谱研究了生物质的表面特性和官能团。结果表明，利用黄孢原毛平革菌从液体介质中同时去除镉和 2,4-DCP 具有很大的应用前景。
