Mitani Noriaki, Koizumi Akira, Sano Reiko, Masutani Takayuki, Murakawa Koichi, Mikasa Keiichi, Okamoto Yasuyuki
Central Clinical Laboratory, Nara Medical University, Nara 634-8522, Japan.
Jpn J Infect Dis. 2005 Aug;58(4):250-2.
A new convenient molecular typing method, simultaneous polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis, for three different genes of methicillin-resistant Staphylococcus aureus (MRSA) was evaluated using 35 isolates of MRSA and comparing results with those previously reported for sequencing-based spa typing. Twenty-nine isolates of the most frequent protein A (spa) type were discriminated into 6 different types by PCR-RFLP. In contrast, spa typing could discriminate only 1 of the 19 most frequent PCR-RFLP-type isolates. The discriminatory powers of the two methods were equal for the other isolates. These results suggest that PCR-RFLP has the advantages of both relative easiness and greater discriminatory power than spa typing. We also report the case of a suspected outbreak in which PCR-RFLP was sufficient for ruling out the possibility of an outbreak. Thus, PCR-RFLP is preferable as a preliminary screening method for epidemiological studies of nosocomial infection caused by MRSA.
一种新的便捷分子分型方法，即同时聚合酶链反应（PCR）-限制性片段长度多态性（RFLP）分析，用于耐甲氧西林金黄色葡萄球菌（MRSA）的三个不同基因，使用35株MRSA分离株进行了评估，并将结果与先前基于测序的spa分型报告的结果进行比较。通过PCR-RFLP，29株最常见蛋白A（spa）型分离株被分为6种不同类型。相比之下，spa分型只能区分19株最常见PCR-RFLP型分离株中的1株。对于其他分离株，两种方法的鉴别力相当。这些结果表明，PCR-RFLP具有相对简便和比spa分型更大鉴别力的优点。我们还报告了一例疑似暴发病例，其中PCR-RFLP足以排除暴发的可能性。因此，PCR-RFLP作为MRSA引起的医院感染流行病学研究的初步筛查方法更可取。
