Evaluation of aminoglycoside modifying enzymes, SCCmec, gene and PCR-RFLP gene typing of isolates from hospitals in Shiraz, southwest of Iran.

is an important human pathogen that causes various infections. Aminoglycosides are broad-spectrum antibiotics used to treat methicillinresistant (MRSA) infections. Typing of isolates by coagulase gene typing and PCR-RFLP gene is a fast and suitable method for epidemiological studies. Aim of the present study was to evaluate the resistance to aminoglycosides, staphylococcal chromosomal cassette mec (SCCmec) types, coagulation typing and PCR-RFLP gene in clinical isolates of . 192 isolates were collected from Namazi and Shahid Faghihi hospitals. Antibiotic resistance was measured by disk diffusion method and MIC was determined for gentamicin. The presence of genes encoding aminoglycoside modifying enzymes () and gene were assessed by PCR. Also the coagulase typing, PCR-RFLP gene, and SCCmec typing were performed. Out of 192 isolated isolates, 83 (43.2%) MRSA isolates were identified. In this study, a high resistance to streptomycin and gentamicin (98.7%) were observed. Among the genes, the gene was the most common. Based on the SCCmec typing, it was determined that the prevalence of SCCmec type III (45.8%) was highest. From the amplification of the gene, 5 different types were obtained. Also, in digestion of gene products by enzyme, 10 different RFLP patterns were observed. According to this study, aminoglycoside resistance is increasing among MRSA isolates. As a result, monitoring and control of aminoglycoside resistance can be effective in the treatment of MRSA isolates. Also, typing of isolates based on gene polymorphism is a suitable method for epidemiological studies.