采用特异性放射免疫分析法测定新生儿尿液中结合型1β-羟基胆酸。

Measurement of conjugated 1 beta-hydroxycholic acid in urine of newborns by specific radioimmunoassay.

作者信息

Ikegawa S, Murai T, Yoshimura T, Xu Z Z, Tohma M

机构信息

Faculty of Pharmaceutical Sciences, Higashi-Nippon-Gakuen University, Hokkaido, Japan.

出版信息

Chem Pharm Bull (Tokyo). 1992 Mar;40(3):701-4. doi: 10.1248/cpb.40.701.

DOI:10.1248/cpb.40.701

PMID:1611682

Abstract

Anti-tauro 1 beta-hydroxycholic acid antisera were prepared by immunizing rabbits with N-(1 beta,3 alpha,7 alpha,12 alpha-tetrahydroxy-5 beta-cholan-24-oyl)glycine bovine serum albumin conjugate. The immunoglobulin G fraction was obtained by ammonium sulfate precipitation, followed by diethylaminoethyl cellulose column chromatography. The antibody was characterized using [2-3H]tauro 1 beta-hydroxycholic acid which has a high affinity (Ka = 1.09 x 10(9) M-1) and reasonable specificity. Cross-reactivity for glyco 1 beta-hydroxycholic acid was 100% and those for other 1 beta-hydroxylated bile acids ranged from 4.32 to 29.6%. Concentrations of conjugated 1 beta-hydroxycholic acid in urine of newborns at 0-20 d after birth were determined by radioimmunoassay to be significant (0.2-11.1 micrograms/ml), exhibiting a tendency to increase during the 20 d after birth.

摘要

通过用N-(1β,3α,7α,12α-四羟基-5β-胆烷-24-酰基)甘氨酸牛血清白蛋白结合物免疫兔子来制备抗牛磺熊去氧胆酸抗血清。通过硫酸铵沉淀，然后进行二乙氨基乙基纤维素柱色谱法获得免疫球蛋白G部分。使用具有高亲和力(Ka = 1.09 x 10(9) M-1)和合理特异性的[2-3H]牛磺熊去氧胆酸对该抗体进行表征。对甘氨熊去氧胆酸的交叉反应性为100%，对其他1β-羟基化胆汁酸的交叉反应性范围为4.32%至29.6%。通过放射免疫测定法测定出生后0至20天新生儿尿液中结合型熊去氧胆酸的浓度具有显著意义(0.2 - 11.1微克/毫升)，在出生后的20天内呈现出增加的趋势。