Kir Hale M, Dillioglugil Meltem O, Tugay Melih, Eraldemir Ceyla, Ozdoğan Haci Kahya
Department of Medical Biochemistry, Faculty of Medicine, Kocaeli University, TR-41050 Kocaeli, Turkey.
Am J Nephrol. 2005 Sep-Oct;25(5):441-6. doi: 10.1159/000087825. Epub 2005 Aug 23.
Excessive generation of reactive oxygen species (ROS) contributes to the process of progressive renal injury in a variety of clinical and experimental renal diseases. The present study was designed to test the hypothesis that treatment with vitamins decreases renal injury in chronic renal failure (CRF).
Forty male Sprague-Dawley rats were divided into 5 groups: group 1, control; group 2, 5/6 nephrectomy (CRF); other groups 5/6 nephrectomy and injected vitamins (E, A, D). After 8 weeks, urea, creatinine and renal tissue malondialdehyde (MDA), glutathione (GSH), nitric oxide (NO) levels and superoxide dismutase (SOD) activities were determined.
Renal tissue MDA levels were significantly lower in the control and Vit E groups compared to that of the CRF, Vit A and Vit D groups. GSH levels were significantly higher in the control group compared to that of other groups. However, GSH levels were significantly lower in the control group than those in the other groups. SOD activities of the control group were significantly higher than those in the other groups. SOD activities were significantly decreased in the Vit E group compared to the Vit A and Vit D groups. Tissue NO levels of control group were significantly increased compared to the other groups.
According to this study, Vit E may at least in part prevent tissue injury by acting as a free radical scavenger.
活性氧（ROS）的过度产生在多种临床和实验性肾脏疾病的进行性肾损伤过程中起作用。本研究旨在验证维生素治疗可减轻慢性肾衰竭（CRF）肾损伤这一假说。
40只雄性Sprague-Dawley大鼠分为5组：第1组为对照组；第2组为5/6肾切除（CRF）组；其他组为5/6肾切除并注射维生素（E、A、D）组。8周后，测定尿素、肌酐以及肾组织丙二醛（MDA）、谷胱甘肽（GSH）、一氧化氮（NO）水平和超氧化物歧化酶（SOD）活性。
与CRF组、维生素A组和维生素D组相比，对照组和维生素E组肾组织MDA水平显著降低。对照组GSH水平显著高于其他组。然而，对照组GSH水平显著低于其他组。对照组SOD活性显著高于其他组。与维生素A组和维生素D组相比，维生素E组SOD活性显著降低。对照组组织NO水平显著高于其他组。
根据本研究，维生素E至少部分可通过作为自由基清除剂来预防组织损伤。
