Pavselj Natasa, Bregar Zvonko, Cukjati David, Batiuskaite Danute, Mir Lluis M, Miklavcic Damijan
Faculty of Electrical Engineering, University of Ljubljana, Ljubljana 1000, Slovenia.
IEEE Trans Biomed Eng. 2005 Aug;52(8):1373-81. doi: 10.1109/TBME.2005.851524.
One of the ways to potentiate antitumor effectiveness of chemotherapeutic drugs is by local application of short intense electric pulses. This causes an increase of the cell membrane permeability and is called electropermeabilization. In order to study the course of tissue permeabilization of a subcutaneous tumor in small animals, a mathematical model was built with the commercial program EMAS, which uses the finite element method. The model is based on the tissue specific conductivity values found in literature, experimentally determined electric field threshold values of reversible and irreversible tissue permeabilization, and conductivity changes in the tissues. The results obtained with the model were then compared to experimental results from the treatment of subcutaneous tumors in mice and a good agreement was obtained. Our results and the reversible and irreversible thresholds used coincide well with the effectiveness of the electrochemotherapy in real tumors where experiments show antitumor effectiveness for amplitudes higher than 900 V/cm ratio and pronounced antitumor effects at 1300 V/cm ratio.
增强化疗药物抗肿瘤效果的方法之一是局部施加短时间的强电脉冲。这会导致细胞膜通透性增加，被称为电穿孔。为了研究小动物皮下肿瘤组织通透化的过程，使用商业软件EMAS建立了一个数学模型，该软件采用有限元方法。该模型基于文献中发现的组织特定电导率值、实验确定的可逆和不可逆组织通透化的电场阈值以及组织中的电导率变化。然后将模型得到的结果与小鼠皮下肿瘤治疗的实验结果进行比较，结果吻合良好。我们的结果以及所使用的可逆和不可逆阈值与实际肿瘤中电化学疗法的有效性非常吻合，实验表明，当电场强度高于900V/cm时具有抗肿瘤效果，而在1300V/cm时具有显著的抗肿瘤作用。
