Watanabe Hirohiko, Nonoguchi Kohsuke, Sakurai Toshiharu, Masuda Tomoko, Itoh Katsuhiko, Fujita Jun
Daigo Watanabe Clinic, Faculty of Medicine, Kyoto University, Kyoto, Japan.
Mol Hum Reprod. 2005 Jul;11(7):471-6. doi: 10.1093/molehr/gah186.
Decidualization of the endometrial stromal cells (ESC), considered to be stimulated by progesterone and/or cAMP, is crucial for embryo implantation and placentation. In this study, we isolated a novel clone encoding decidual protein induced by progesterone (Depp) from a human ESC cDNA library enriched with progesterone-inducible genes. Depp mRNA was expressed in various human tissues including placenta, ovary and kidney. Increased expression of Depp was observed in endometria during mid- and late-secretory phases and 1st trimester deciduas. In vitro, Depp mRNA was induced in ESC within 30 min of progesterone treatment, which was inhibited by the antiprogestin RU486. Androgen alone also induced Depp expression. Depp increased the level of phosphorylated Erk and activated the Elk-1 transcription factor in human embryonal kidney 293 cells, suggesting that Depp modulates the effects of progesterone during decidualization and in the decidua by affecting gene expression. Elucidation of the biological function of Depp in the endometrium will facilitate our understanding of the molecular mechanisms of decidualization and placental development.
子宫内膜基质细胞（ESC）的蜕膜化被认为是由孕酮和/或环磷酸腺苷（cAMP）刺激引起的，对胚胎着床和胎盘形成至关重要。在本研究中，我们从富含孕酮诱导基因的人ESC cDNA文库中分离出一个编码孕酮诱导蜕膜蛋白（Depp）的新克隆。Depp mRNA在包括胎盘、卵巢和肾脏在内的多种人体组织中表达。在分泌中期和晚期子宫内膜以及妊娠早期蜕膜中观察到Depp表达增加。在体外，孕酮处理30分钟内ESC中诱导Depp mRNA表达，这被抗孕激素RU486抑制。单独雄激素也诱导Depp表达。Depp增加人胚肾293细胞中磷酸化细胞外信号调节激酶（Erk）水平并激活Elk-1转录因子，表明Depp通过影响基因表达在蜕膜化过程中和蜕膜中调节孕酮的作用。阐明Depp在子宫内膜中的生物学功能将有助于我们理解蜕膜化和胎盘发育的分子机制。
