Ekiert H, Czygan F-Ch
Chair and Department of Pharmaceutical Botany, Collegium Medicum, Jagiellonian University, 9 Medyczna Street, 30-688 Kraków, Poland.
Pharmazie. 2005 Aug;60(8):623-6.
This study was designed to investigate the dynamics of accumulation of linear furanocoumarins (psoralen, bergapten, xanthotoxin, isopimpinellin, imperatorin) and their biogenetic precursor, umbelliferone, in agitated cultures of Ruta graveolens L. and Ruta graveolens ssp. divaricata (Tenore) Gams during 6-week growth cycles. The metabolites under study were almost exclusively accumulated in the cultured biomass. The total content of all metabolites increased 4.8- and 2.0-fold, in R. graveolens and R. graveolens ssp. divaricata cultures, respectively. Xanthotoxin and bergapten, which are the most important therapeutic compounds, were the dominating metabolites in cultures of both plants. The maximum content of xanthotoxin (25.0 mg/100 g dry wt.) and bergapten (18.4 mg/100 g dry wt) and the maximum content of all metabolites (64.0 mg/100 g dry wt) in R. graveolens ssp. divaricata callus obtained on the 35th culture day were relatively low. However, maximum contents of xanthotoxin (136.8 mg/100 g dry wt), bergapten (210.4 mg/100 g dry wt.) and isopimpinellin (96.7 mg/100 g dry wt), and total content of all metabolites in R. graveolens shoots (520.8 mg/100 g dry wt) obtained on the 42nd culture day are interesting from a practical point of view.
本研究旨在调查在6周生长周期内，线性呋喃香豆素（补骨脂素、佛手柑内酯、花椒毒素、异茴芹素、欧前胡素）及其生物合成前体伞形花内酯在芸香和裂叶芸香悬浮培养物中的积累动态。所研究的代谢产物几乎完全积累在培养的生物量中。在芸香和裂叶芸香培养物中，所有代谢产物的总含量分别增加了4.8倍和2.0倍。花椒毒素和佛手柑内酯是最重要的治疗性化合物，在两种植物的培养物中都是主要的代谢产物。在第35个培养日获得的裂叶芸香愈伤组织中，花椒毒素的最大含量（25.0毫克/100克干重）和佛手柑内酯的最大含量（18.4毫克/100克干重）以及所有代谢产物的最大含量（64.0毫克/100克干重）相对较低。然而，从实际角度来看，在第42个培养日获得的芸香嫩枝中，花椒毒素的最大含量（136.8毫克/100克干重）、佛手柑内酯的最大含量（210.4毫克/100克干重）和异茴芹素的最大含量（96.7毫克/100克干重）以及所有代谢产物的总含量（520.8毫克/100克干重）很有意思。
