在非生物诱导子存在下积累的芸香代谢产物的鉴定。

Identification of Ruta graveolens L. metabolites accumulated in the presence of abiotic elicitors.

作者信息

Orlita Aleksandra, Sidwa-Gorycka Matylda, Kumirska Jolanta, Maliński Edmund, Siedlecka Ewa M, Gajdus Jerzy, Lojkowska Ewa, Stepnowski Piotr

机构信息

Faculty of Chemistry, University of Gdańsk, PL 80-952 Gdańsk, ul. Sobieskiego 18, Gdańsk, Poland.

出版信息

Biotechnol Prog. 2008 Jan-Feb;24(1):128-33. doi: 10.1021/bp070261d. Epub 2007 Dec 6.

DOI:10.1021/bp070261d

PMID:18052337

Abstract

The study aimed to elucidate the effects of benzothiadiazole (BTH) and saccharin on the biosynthesis of simple coumarins, linear furanocoumarins, dihydrofuranocoumarins, and furoquinolone alkaloids in shoots of R. graveolens cultivated in vitro. The biosynthesized metabolites were analyzed and identified by GC-MS and by comparison of Kovats indices. Eight coumarin metabolites were identified: bergapten, chalepin, isopimpinelin, pinnarin, psoralen, rutacultin, rutamarin, and xanthotoxin, and also four alkaloids: dictamnine, gamma-fagarine, skimmianine, and kokusaginine. Each of the tested BTH concentrations induced a significant production of furanocoumarins and furoquinolone alkaloids. The use of saccharin also increased the production of bergapten, isopimpinelin, pinnarin, psoralen, and xanthotoxin several times.

摘要

该研究旨在阐明苯并噻二唑（BTH）和糖精对体外培养的芸香枝条中简单香豆素、线性呋喃香豆素、二氢呋喃香豆素和呋喃喹啉生物碱生物合成的影响。通过气相色谱-质谱联用（GC-MS）以及比较保留指数对生物合成的代谢产物进行分析和鉴定。鉴定出了8种香豆素代谢产物：补骨脂素、白藓素、异茴芹内酯、紫花前胡内酯、补骨脂内酯、芸香苦素、芸香亭和花椒毒素，以及4种生物碱：白藓碱、γ-崖椒碱、茵芋碱和黄柏碱。每种测试的BTH浓度均显著诱导了呋喃香豆素和呋喃喹啉生物碱的产生。使用糖精也使补骨脂素、异茴芹内酯、紫花前胡内酯、补骨脂内酯和花椒毒素的产量提高了数倍。