Diwan Renuka, Malpathak Nutan
Department of Botany, University of Pune, India.
Nat Prod Commun. 2012 Jul;7(7):895-8.
The influence of the polyamines putrescine (Put), spermine (Spr) and spermidine (Spd) on growth and furanocoumarin production was investigated by exogenous addition, at different concentrations, to shoot cultures of Ruta graveolens at different phases of growth. Preliminary studies indicated that addition of Put (20 microM) and Spr (80 microM) had a promotive effect on shoot multiplication rate and number of multiple shoots formed. Spd was toxic, even at lower concentrations. The growth-phase of the culture at the time of exogenous addition of polyamines was found to be an important factor. Put was most effective when added at the lag phase, while Spr was most effective when added in the log phase. Time course studies of growth and furanocoumarin content were carried out for each polyamine and phase of addition. It was seen that maximum production of furanocoumarins (256.8 mg/10 g DW) occurred in the second week when Put was added in the lag phase and 260.5 mg/10 g DW in the fourth week when Spr was added in the log phase. Put addition resulted in a 3.10 fold increase in psoralen, 6.12 in xanthotoxin and 1.46 fold in bergapten production. Spr addition resulted in a 1.31 fold increase in psoralen, 4.11 fold in xanthotoxin and 1.49 fold in bergapten production. Results indicate that alteration of growth and furanocoumarin production kinetics is a combined outcome of choice of polyamine and the phase of culture at the time of exogenous addition. Polyamine addition enabled significant enhancement in production of pharmaceutically important bergapten and xanthotoxin in shoot cultures of Ruta graveolens, which could be explored for commercial production.
通过在不同生长阶段向芸香的茎段培养物中外源添加不同浓度的多胺腐胺（Put）、精胺（Spr）和亚精胺（Spd），研究了它们对生长和呋喃香豆素产生的影响。初步研究表明，添加Put（20 microM）和Spr（80 microM）对茎段增殖率和形成的丛生芽数量有促进作用。即使在较低浓度下，Spd也具有毒性。发现外源添加多胺时培养物的生长阶段是一个重要因素。在延迟期添加Put最有效，而在对数期添加Spr最有效。对每种多胺及其添加阶段进行了生长和呋喃香豆素含量的时间进程研究。可以看出，当在延迟期添加Put时，呋喃香豆素的最大产量（256.8 mg/10 g干重）出现在第二周，当在对数期添加Spr时，在第四周为260.5 mg/10 g干重。添加Put导致补骨脂素产量增加3.10倍，花椒毒素增加6.12倍，佛手柑内酯增加1.46倍。添加Spr导致补骨脂素产量增加1.31倍，花椒毒素增加4.11倍，佛手柑内酯增加1.49倍。结果表明，生长和呋喃香豆素产生动力学的改变是多胺选择和外源添加时培养阶段共同作用的结果。添加多胺能够显著提高芸香茎段培养物中具有重要药用价值的佛手柑内酯和花椒毒素的产量，这可用于商业生产探索。
