[炮制对大黄蒽醌含量的影响]

[Influence of processing rhubarb on it's anthraquinone contents].

作者信息

Li Xian-Duan, Huang Lu-Qi

机构信息

Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, BeJing 100700, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2005 Jun;30(12):904-6, 943.

PMID:16124606

Abstract

OBJECTIVE

To investigate the influence of processing of Radix et Rhizoma Rhei on it's five anthraquinone components, and to develop a HPLC quantatitive method for these components in Radix et Rhizoma Rhei.

METHOD

Chromatographic conditions are as follow: Kromasil-C18 (4.6 mmx 150 mm, 5 microm) column, ethanol -H2O-H3PO4 (85:15:0.1) as mobile phase and the detection wavelength at 254 nm.

RESULT

The average recoveries are 97.9% (aleo-emodin), 97.1% (rhein), 97.6% (emodin), 97.4% (chrysophanol) and 99.1% (physcion). RSD are 1.4%, 1.1%, 0.9%, 1.1% and 2.2% correspondingly. After processing, the contents of five anthraquinone aglycones descended compared with the crude material, the descending percentage are 15.9% (aleo-emodin), 28.0% (rhein), 25.8% (emodin), 10.0% (chrysophanol) and 10.3% (physcion).

CONCLUSION

This method is simple, sensitive and repeatable. It' canbeased for quality control of Radix et Rhizoma Rhei and it's processed products.

摘要

目的

考察大黄炮制对其5种蒽醌类成分的影响，建立大黄中这些成分的高效液相色谱定量分析方法。

方法

色谱条件如下：Kromasil-C18（4.6 mm×150 mm，5μm）柱，以乙醇-水-磷酸（85:15:0.1）为流动相，检测波长为254 nm。

结果

平均回收率分别为：芦荟大黄素97.9%、大黄酸97.1%、大黄素97.6%、大黄酚97.4%、大黄素甲醚99.1%。相对标准偏差分别为1.4%、1.1%、0.9%、1.1%和2.2%。炮制后，5种蒽醌苷元的含量均较生品有所下降，下降率分别为：芦荟大黄素15.9%、大黄酸28.0%、大黄素25.8%、大黄酚10.0%、大黄素甲醚10.3%。