Robertson N G, Morton C C
Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts 02115.
Genomics. 1992 Jun;13(2):449-51. doi: 10.1016/0888-7543(92)90269-x.
The myc family of proto-oncogenes consists of several members that possess regions of sequence homology and some have known similarities in structure and function. We have isolated an 8.8-kb EcoRI fragment from a human genomic library by hybridization to a 28-base oligonucleotide probe derived from a region of the second exon of MYC, which is highly conserved in the myc gene family. Sequence analysis of this myc-like (MYCLK1) DNA fragment has revealed the existence of a region with 85% homology to the 28-base oligonucleotide probe. An open reading frame of 207 nucleotides containing the region of homology was found. We have mapped MYCLK1 to human chromosome 7 at band p15 by chromosome in situ hybridization; this site is distinct from the map location of previously characterized myc genes. Whether MYCLK1 represents a new functional member of the myc family of proto-oncogenes remains to be determined.
原癌基因的myc家族由几个成员组成，这些成员具有序列同源区域，并且有些在结构和功能上具有已知的相似性。我们通过与一个28碱基的寡核苷酸探针杂交，从人类基因组文库中分离出一个8.8kb的EcoRI片段，该探针来源于MYC第二个外显子的一个区域，此区域在myc基因家族中高度保守。对这个类myc（MYCLK1）DNA片段的序列分析揭示了一个与28碱基寡核苷酸探针具有85%同源性的区域的存在。发现了一个包含同源区域的207个核苷酸的开放阅读框。我们通过染色体原位杂交将MYCLK1定位到人类染色体7的p15带；这个位点与先前鉴定的myc基因的图谱位置不同。MYCLK1是否代表原癌基因myc家族的一个新的功能成员仍有待确定。
