Qiu Yan, Yang Hai-Ping, Zha Yi, Li Li-Li, Li Su-Bo, Wang De-Hai, Zhang Zi-Xin, Zhang Yang-Pei
Beijing Red Cross Blood Center, Beijing 100088, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2005 Aug;13(4):692-7.
In order to study whether plasma can affect the structure and function of red blood cells during their storage period, the differences of pH value, concentration of K(+), Na(+), osmotic fragility, plasma hemoglobin, AchE, ATP, 2.3-DPG, P50 in suspended RBC, washed RBC, and RBC with various plasma volume at different storage times were compared. The results showed that plasma helped the blood to keep the RBC at high pH value, low K(+), high Na(+) and maintain RBC-ATP, oxygen carry capacity and deformability, but no effect on maintenance of osmotic fragility, and levels of plasma hemoglobin, AchE, ATP and 2.3-DPG was found in preservated blood. In conclusion, human plasma may be in favour of the preservation of red blood cells.
为研究血浆在红细胞保存期间是否会影响其结构和功能，比较了悬浮红细胞、洗涤红细胞以及不同血浆量的红细胞在不同保存时间下的pH值、K(+)、Na(+)浓度、渗透脆性、血浆血红蛋白、乙酰胆碱酯酶（AchE）、三磷酸腺苷（ATP）、2,3-二磷酸甘油酸（2.3-DPG）、P5(0)的差异。结果显示，血浆有助于血液使红细胞保持高pH值、低钾(+)、高钠(+)，并维持红细胞ATP、氧携带能力及变形性，但对维持渗透脆性无影响，且在保存血液中未发现血浆血红蛋白、AchE、ATP及2.3-DPG水平有变化。总之，人血浆可能有利于红细胞的保存。
