Mortier Leen, Huet Anne-Catherine, Daeseleire Els, Huyghebaert Gerard, Fodey Terrence, Elliott Christopher, Delahaut Philippe, Van Peteghem Carlos
Ministry of the Flemish Community, Agricultural Research Centre Ghent (CLO), Department of Animal Product Quality and Transformation Technology (DVK), Brusselsesteenweg 370, 9090 Melle, Belgium.
J Agric Food Chem. 2005 Sep 7;53(18):7142-9. doi: 10.1021/jf050836r.
Anticoccidials are compounds that are widely used as feed additives to prevent and treat coccidiosis. They are licensed for use in a prescribed concentration and during a certain time interval for broilers and pullets but not for laying hens. It was shown in the past that carry-over at the feeding mill is found to be the main reason for the presence of residues in eggs. An animal experiment was set up to investigate the effect of carry-over at the feeding mill on the presence of residues of anticoccidials in eggs. For the compounds diclazuril, robenidine, halofuginone and nicarbazin in combination with narasin, two concentration levels were tested: the maximum allowed concentration for broilers (100%) and a concentration corresponding to 5% carry-over during feed preparation. Also dimetridazole was included in the experiment but only at one concentration level. Eggs were sampled during treatment (14 days) and for a period of 30 days after withdrawal of the anticoccidial-containing feed. Residues were determined, and deposition and depletion curves were generated. Analyses were performed by ELISA and LC-MS/MS. For all compounds, substantial residues could be found in the 5% groups, which points out the risk of carry-over at the feeding mill. The distribution of the residues between egg yolk and white was determined by analyzing both fractions.
抗球虫药是广泛用作饲料添加剂以预防和治疗球虫病的化合物。它们被许可在规定浓度和特定时间间隔内用于肉鸡和小母鸡，但不适用于产蛋母鸡。过去的研究表明，饲料厂的残留是鸡蛋中出现残留的主要原因。为此进行了一项动物实验，以研究饲料厂的残留对鸡蛋中抗球虫药残留的影响。对于地克珠利、氯苯胍、常山酮和尼卡巴嗪与甲基盐霉素的组合化合物，测试了两个浓度水平：肉鸡的最大允许浓度（100%）和饲料制备过程中相当于5%残留的浓度。实验中还包括二甲硝唑，但仅在一个浓度水平。在治疗期间（14天）以及停用含抗球虫药饲料后的30天内采集鸡蛋样本。测定残留量，并绘制沉积和消除曲线。采用酶联免疫吸附测定法（ELISA）和液相色谱-串联质谱法（LC-MS/MS）进行分析。对于所有化合物，在5%组中均发现大量残留，这表明饲料厂存在残留风险。通过分析蛋黄和蛋清两个部分来确定残留量在蛋黄和蛋清之间的分布。
