[Effects of the glucosidorum tripterygll totorum on the proliferation and apoptosis of cell line HL-60 in human acute promyelocytic leukemia].

OBJECTIVE

To determine the effects of the glucosidorum tripterygll totorum on the proliferation inhibition and apoptosis induction of cell line HL-60 in human acute promyelocytic leukemia.

METHODS

The survival rates of cells were tested by MTT assay, fluorescence stain, flow cytometry test, and DNA fragmentation.

RESULTS

Glucosidorum tripterygll totorum could notably reduce the survival rate of HL-60 cells, and inhibit the proliferation of tumor cells. The 50% inhibitory concentration (IC50) was 5.0 microg/ml and it arrested the cell cycle at G2/M phase (P < 0. 05). The typical apoptosis morphologic cells were seen, the rates of apoptosis cells were increased (P < 0.05), and DNA ladder appeared in the experiment group.

CONCLUSION

Glucosidorum tripterygll totorum could notably inhibit the proliferation and induce the apoptosis of HL-60 cells.