Mi Zu-Xia, Xie Qi-Yang
Stem Cell Research Center, Hainan Medical College, Haikou 570102, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2004 Aug;29(4):393-6.
To set up a stable technology for the growth of yolk sac CFU-GM.
The effects of various inductive factors on the growth of yolk sac CFU-GM and the characteristic of colonies were investigated. In vitro agar culture method was used in this study.
We found close relationship between the number of E7.5 - E8.5 yolk sac cells planted and the number of the formation of CFU-GM colonies. The number of the formation of CFU-GM colonies in DMEM culture system was higher than that in the IMDM culture system with the same number of yolk sac cells planted. The effect of HS culture system on the formation of CFU-GM colonies was better than that of FBS culture system. In contrast with GM-CSF, WEHI-3-CM had much more significant CFU-GM growth promoting effect (4.14 times improved, P < 0.01). The colonies derived from yolk sac cells in this study were CFU-GM colonies by in situ fixation and H. E staining.
The committed differentiation from yolk sac stem cells to CFU-GM is affected by many factors. The combination of DMEM, HS, GM-CSF or WEHI-3-CM in culture system is optimal for yolk sac CFU-GM growth.
建立稳定的卵黄囊CFU-GM生长技术。
研究各种诱导因素对卵黄囊CFU-GM生长及集落特性的影响。本研究采用体外琼脂培养法。
我们发现接种的E7.5 - E8.5卵黄囊细胞数量与CFU-GM集落形成数量之间存在密切关系。在接种相同数量卵黄囊细胞的情况下，DMEM培养体系中CFU-GM集落形成数量高于IMDM培养体系。HS培养体系对CFU-GM集落形成的效果优于FBS培养体系。与GM-CSF相比，WEHI-3-CM对CFU-GM生长的促进作用更为显著（提高了4.14倍，P < 0.01）。通过原位固定和苏木精-伊红染色证实本研究中源自卵黄囊细胞的集落为CFU-GM集落。
卵黄囊干细胞向CFU-GM的定向分化受多种因素影响。培养体系中DMEM、HS、GM-CSF或WEHI-3-CM的组合对卵黄囊CFU-GM生长最为适宜。
