Anckaert M A, Symann M
J Embryol Exp Morphol. 1983 Feb;73:87-95.
In order to evaluate the hypothetical activity of foetal hepatic factors on putative yolk-sac haemopoietic stem cells we used the Double Diffusion Chamber (DDC) techniqUe. The DDC were made of a regulator compartment, where foetal hepatic tissue was introduced and a test compartment where visceral yolk-sac cells were cultured. In this system a hepatic signal induced the yolk-sac stem cells to differentiate along the granulocytic pathway but did not stimulate yolk-sac CFUs growth. Contrary to CFUs originating from foetal liver or adult bone marrow, yolk-sac CFUs do not increase numerically in diffusion chamber culture.
为了评估胎儿肝脏因子对假定的卵黄囊造血干细胞的假定活性，我们使用了双扩散室（DDC）技术。双扩散室由一个调节室和一个测试室组成，调节室中引入胎儿肝脏组织，测试室中培养内脏卵黄囊细胞。在这个系统中，肝脏信号诱导卵黄囊干细胞沿粒细胞途径分化，但不刺激卵黄囊集落形成单位（CFUs）的生长。与源自胎儿肝脏或成人骨髓的CFUs相反，卵黄囊CFUs在扩散室培养中数量不会增加。
