Li Jing
Department of Forensic Medicine, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2004 Aug;29(4):432-4.
To explore the application of paternity identification by chromosome STR genetic markers (short tandem repeat).
The paternity testings in 468 cases were routinely carried out using amphibian STR Profiler plus and Cofiler PCR amplification kits. When STR exclusions were found, HLA system and other blood groups were detected by molecular typing and loci of PowerPlex 16 system. STR loci were genotyped. If necessary, the genotyping of Y chromosome specific STR, X chromosome specific STR and HLA allelic sequencing were added.
W of 408 cases of paternity was more than 0.95, and that of 350 cases was more than 0. 9995. W of 60 cases of unrelated individuals was less than 0.8, and that of another 48 cases was less than 0.27. By Chi-Square test, there was significant difference (P < 0.001) in the entire-same allelic genes and the entire-different allelic genes and no significant difference (P > 0.05) in the half-same between allelic genes the two groups.
It is effective to identify the paternity by STR genetic markers. The pair is related when the locus number of entirely-different allelic genes is less than 1 or that of the entirely-same allelic genes is more than 6.
探讨染色体短串联重复序列（STR）基因标记在亲子鉴定中的应用。
应用AmpliFiler Plus和Cofiler PCR扩增试剂盒对468例亲子鉴定案例进行常规检测。当发现STR排除时，采用分子分型检测HLA系统及其他血型，并检测PowerPlex 16系统位点。对STR位点进行基因分型。必要时，增加Y染色体特异性STR、X染色体特异性STR基因分型及HLA等位基因测序。
408例亲子鉴定案例中父权指数（W）大于0.95，350例大于0.9995。60例无关个体的W小于0.8，另48例小于0.27。经卡方检验，两组等位基因全相同和全不同的差异有统计学意义（P<0.001），等位基因半相同的差异无统计学意义（P>0.05）。
STR基因标记用于亲子鉴定是有效的。当全不同等位基因位点数小于1或全相同等位基因位点数大于6时，二者具有亲缘关系。
