[中国鄂伦春族Y染色体短串联重复序列的遗传多态性]
[Genetic polymorphism of Y-chromosome short tandem repeat in Elunchun ethnic group of China].
作者信息
Feng Xue, Xu Ping, Li Sheng-bin
机构信息
The Key Laboratory of National Ministry of Health for Forensic Science, Xi'an Jiaotong University, Xi'an, Shaanxi, PR China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2005 Oct;22(5):585-8.
OBJECTIVE
To reveal the allelic frequencies and haplotype frequencies of ten Y-chromosome short tandem repeats(STR) loci (DYS392, DYS438, DYS439, DYS456, DYS459, DYS460,DYS461, DYS462, DYS389I and DYS389II systems) in an Elunchun population sample.
METHODS
PCR and polyacrylamidegel electrophoresis(PAGE) followed by silver staining were performed to analyze the blood samples from 102 unrelated Elunchun male individuals.
RESULTS
The allele diversity values for each locus ranged from 0.418(DYS461) to 0.727(DYS389 I). Gene diversities were higher than 0.5 except DYS462(0.479) and DYS461 (GATAA7.2)(0.418). Furthermore, 101 haplotypes from 10 Y-chromosome STRs loci were found in the sample and a high haplotype diversity 0.99 was determined.
CONCLUSION
The above findings suggest that the ten Y-chromosome STR loci are valuable Y-specific markers for establishing Y-STR database, understanding human origin, paternity testing and personal identification.
目的
揭示鄂伦春族群体样本中10个Y染色体短串联重复序列（STR）基因座（DYS392、DYS438、DYS439、DYS456、DYS459、DYS460、DYS461、DYS462、DYS389I和DYS389II系统）的等位基因频率和单倍型频率。
方法
采用聚合酶链反应（PCR）及聚丙烯酰胺凝胶电泳（PAGE）结合银染技术，对102名无血缘关系的鄂伦春族男性个体的血样进行分析。
结果
各基因座的等位基因多样性值范围为0.418（DYS461）至0.727（DYS389I）。除DYS462（0.479）和DYS461（GATAA7.2）（0.418）外，基因多样性均高于0.5。此外，在样本中发现了10个Y染色体STR基因座的101种单倍型，单倍型多样性高达0.99。
结论
上述结果表明，这10个Y染色体STR基因座是建立Y-STR数据库、了解人类起源、进行亲子鉴定和个人识别的有价值的Y特异性标记。
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