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将五种哺乳动物细胞系和一种蚊子细胞系在无血清冷冻保存剂中附着于培养瓶时，于-80摄氏度下进行快速冷冻保存。

Rapid cryopreservation of five mammalian and one mosquito cell line at -80 degrees C while attached to flasks in a serum free cryopreservative.

作者信息

Corsini Joe, Mann Ethan

机构信息

Chadron State College, Chadron, NE 69337, USA.

出版信息

Biol Proced Online. 2005;7:26-30. doi: 10.1251/bpo102. Epub 2005 Mar 14.

DOI:10.1251/bpo102
PMID:16136221
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1190376/
Abstract

Cell culturing, and the requisite storage of cell lines at ultra-low temperatures, is used in most laboratories studying or using eukaryotic proteomics, genomics, microarray, and RNA technologies. In this study we have observed that A72(dog), CRFK(cat), NB324K(human), MCF7(human), WI38(human), and C636(mosquito) cells were effectively cryopreserved at -80 degrees C while attached to the substratum of 25 cm2 tissue culture flasks. This was accomplished using a serum free crypreservative recently developed by Corsini and co-workers. The technique allows for significant savings of time and money in laboratories that rapidly process numerous cell lines.

摘要

细胞培养以及在超低温下对细胞系进行必要的保存，在大多数研究或使用真核蛋白质组学、基因组学、微阵列和RNA技术的实验室中都会用到。在本研究中，我们观察到A72（狗）、CRFK（猫）、NB324K（人）、MCF7（人）、WI38（人）和C636（蚊子）细胞在附着于25 cm²组织培养瓶的基质上时，能在-80℃下有效冷冻保存。这是使用Corsini及其同事最近开发的一种无血清冷冻保护剂实现的。该技术可为快速处理大量细胞系的实验室节省大量时间和金钱。

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Serum-Free Cryopreservation of Five Mammalian Cell Lines in Either a Pelleted or Suspended State.五种哺乳动物细胞系在沉淀或悬浮状态下的无血清冷冻保存
Biol Proced Online. 2004;6:61-66. doi: 10.1251/bpo73. Epub 2004 Apr 7.
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