Wells D E, Price P J
J Immunol Methods. 1983 Apr 15;59(1):49-52. doi: 10.1016/0022-1759(83)90144-8.
Macroscopic hybridoma colonies were frozen and recovered in a good state of viability in 96-well microculture plates using 2 freezing procedures. These methods offer convenient and rapid means of preserving hybridomas and will permit laboratories developing monoclonal antibodies to distribute workloads to more manageable levels without discarding possibly valuable hybridomas.
使用两种冷冻方法将肉眼可见的杂交瘤集落冷冻，并在96孔微量培养板中以良好的活力复苏。这些方法为保存杂交瘤提供了方便快捷的手段，将使开展单克隆抗体制备的实验室能够将工作量分配到更易于管理的水平，而无需丢弃可能有价值的杂交瘤。
