Weidenbaum M, Foster R J, Best B A, Saed-Nejad F, Nickoloff E, Newhouse J, Ratcliffe A, Mow V C
Department of Orthopaedic Surgery, Columbia University, New York, New York.
J Orthop Res. 1992 Jul;10(4):552-61. doi: 10.1002/jor.1100100410.
Magnetic resonance imaging was used to determine the T2 relaxation times of prepared proteoglycan solutions and of normal human intervertebral disc tissue from the annulus fibrosus (AF) and nucleus pulposus (NP). The collagen, proteoglycan, and water contents of the disc tissue samples were determined by biochemical assays after they were scanned. Correlations among 1/T2, collagen, proteoglycan, and water contents of the tissue samples and among 1/T2, water, and proteoglycan contents of the proteoglycan solutions were calculated. A moderate negative correlation between 1/T2 and water content was noted for the tissue samples, and a very high negative correlation was found between 1/T2 and water content for the proteoglycan solutions. The very high positive correlation between 1/T2 and proteoglycan content of the proteoglycan solutions is probably due to this negative correlation between 1/T2 and water content. There was no significant correlation between 1/T2 and proteoglycan content of the tissues. The moderate positive correlation between 1/T2 and collagen content is probably due to the high negative correlation between collagen content and water content. No significant correlation was found between the collagen and proteoglycan contents of the tissues. Thus it appears that the data confirm previous reports in the literature that the collagen of the disc tissue functions to control its water content.
采用磁共振成像技术测定制备的蛋白聚糖溶液以及来自纤维环（AF）和髓核（NP）的正常人椎间盘组织的T2弛豫时间。在对椎间盘组织样本进行扫描后，通过生化分析测定其胶原蛋白、蛋白聚糖和水含量。计算组织样本的1/T2、胶原蛋白、蛋白聚糖和水含量之间以及蛋白聚糖溶液的1/T2、水和蛋白聚糖含量之间的相关性。对于组织样本，观察到1/T2与水含量之间存在中度负相关，而对于蛋白聚糖溶液，发现1/T2与水含量之间存在非常高的负相关。蛋白聚糖溶液的1/T2与蛋白聚糖含量之间非常高的正相关可能归因于1/T2与水含量之间的这种负相关。组织的1/T2与蛋白聚糖含量之间无显著相关性。1/T2与胶原蛋白含量之间的中度正相关可能归因于胶原蛋白含量与水含量之间的高负相关。未发现组织的胶原蛋白和蛋白聚糖含量之间存在显著相关性。因此，数据似乎证实了文献中先前的报道，即椎间盘组织中的胶原蛋白起到控制其水含量的作用。
