Säämänen A M, Puustjärvi K, Ilves K, Lammi M, Kiviranta I, Jurvelin J, Helminen H J, Tammi M
Department of Anatomy, University of Kuopio, Finland.
Int J Sports Med. 1993 Jan;14(1):48-51. doi: 10.1055/s-2007-1021145.
Collagen and proteoglycans in the intervertebral disc (LI-II) of young beagle dogs (age 55 weeks) were analyzed following a 15 weeks' daily 20 km running training on a treadmill with 15 degree uphill inclination. In nucleus pulposus no statistically significant alterations were found in the content of proteoglycans or collagen. In annulus fibrosus the total tissue wet weight and total amount of collagen (hydroxyproline) increased by 34-36% in the runners as compared to age-matched, untrained controls. Since the total amount of proteoglycans did not increase, the annulus fibrosus became relatively depleted of proteoglycans, as indicated by the 27% reduction in uronic acid concentration, expressed either per wet weight or hydroxyproline. The average molecular size of the remaining nonaggregating proteoglycans was larger, and there was also a trend towards increased proportion of proteoglycans aggregating with hyaluronan. Most of the chondroitin sulfate side chains were 6-sulfated (65-66%). Running did not alter the sulfation or length of the chondroitin sulfate chains. The decreased proteoglycan/collagen ratio in annulus fibrosus may result in altered mechanical properties of the tissue and reflects its adaptation to enhanced motion and stress.
对55周龄的幼年比格犬椎间盘（L1-L2）中的胶原蛋白和蛋白聚糖进行了分析，这些犬在跑步机上进行了为期15周、每天20公里、坡度为15度上坡的跑步训练。在髓核中，未发现蛋白聚糖或胶原蛋白含量有统计学上的显著变化。在纤维环中，与年龄匹配的未训练对照组相比，跑步组的组织总湿重和胶原蛋白（羟脯氨酸）总量增加了34%-36%。由于蛋白聚糖总量没有增加，纤维环中的蛋白聚糖相对减少，以每湿重或羟脯氨酸计算的糖醛酸浓度降低了27%就表明了这一点。剩余的非聚集性蛋白聚糖的平均分子大小更大，并且与透明质酸聚集的蛋白聚糖比例也有增加的趋势。大多数硫酸软骨素侧链是6-硫酸化的（65%-66%）。跑步并未改变硫酸软骨素链的硫酸化或长度。纤维环中蛋白聚糖/胶原蛋白比例的降低可能会导致组织力学性能的改变，并反映其对增强运动和压力的适应性。
