Wyss A, von Stockar U, Marison I W
Laboratory of Chemical and Biochemical Engineering, Swiss Federal Institute of Technology, EPFL, CH-1015 Lausanne, Switzerland.
Biotechnol Bioeng. 2006 Jan 5;93(1):28-39. doi: 10.1002/bit.20687.
A novel reactive perstraction system has been developed based on liquid-core capsules, involving an enzyme-catalyzed reaction coupled with simultaneous in situ product recovery. Lipase-catalyzed reactions, hydrolysis of triprionin and nitrophenyl laurate, were selected to test the system and demonstrate the feasibility of immobilization of enzymes to the membranes of liquid-core capsules and the ability to extract hydrophobic products of the reaction within the capsule core. The lipase from Candida rugosa was immobilized to the microcapsules by adsorption and by covalent binding through activation with glutaraldehyde. In both cases improved temperature and operational stability were achieved. Both types of immobilization resulted in a basic shift of the pH optimum for activity, from 7.5 to 9.0. The presence of an organic phase within the capsule core allowed direct product separation and lead to a decrease in product inhibition of the lipase-catalyzed reaction.
基于液芯胶囊开发了一种新型反应萃取系统，该系统涉及酶催化反应以及同时进行的原位产物回收。选择脂肪酶催化的反应，即三软脂精和月桂酸对硝基苯酯的水解反应，来测试该系统，并证明将酶固定在液芯胶囊膜上的可行性以及在胶囊核心内萃取反应疏水产物的能力。通过吸附以及用戊二醛活化后共价结合的方式，将皱褶假丝酵母脂肪酶固定在微胶囊上。在这两种情况下，都实现了温度和操作稳定性的提高。两种固定化方式均导致活性的最适pH值从7.5碱性转变为9.0。胶囊核心内有机相的存在使得产物能够直接分离，并减少了脂肪酶催化反应的产物抑制作用。
