响应曲面法研究皱褶假丝酵母脂肪酶在聚（γ-谷氨酸）上共价固定化的最佳条件
Studies of optimum conditions for covalent immobilization of Candida rugosa lipase on poly(gamma-glutamic acid) by RSM.
作者信息
Chang S-W, Shaw J-F, Yang K-H, Chang S-F, Shieh C-J
机构信息
Department of Nutrition and Health Science, Chung-Chou University of Technology, No.6, Lane 2, Sec.3, Shanjiao Rd. Yuan-Lin, Chang-Hua 503, Taiwan.
出版信息
Bioresour Technol. 2008 May;99(8):2800-5. doi: 10.1016/j.biortech.2007.06.020. Epub 2007 Aug 13.
Poly(gamma-glutamic acid) (gamma-PGA) is a material of polymer. Immobilization of Candida rugosa lipase (Lipase AY-30) by covalent binding on gamma-PGA led to a markedly improved performance of the enzyme. Response surface methodology (RSM) and 3-level-3-factor fractional factorial design were employed to evaluate the effects of immobilization parameters, such as immobilization time (2-6h), immobilization temperature (0-26 degrees C), and enzyme/support ratio (0.1-0.5, w/w). Based on the analysis of ridge max, the optimum immobilization conditions were as follows: immobilization time 2.3h, immobilization temperature 13.3 degrees C, and enzyme/support ratio 0.41 (w/w); the highest lipase activity obtained was 1196 U/mg-protein.
聚（γ-谷氨酸）（γ-PGA）是一种聚合物材料。通过共价结合将皱褶假丝酵母脂肪酶（脂肪酶AY-30）固定在γ-PGA上，可显著提高该酶的性能。采用响应面法（RSM）和三水平三因素部分因子设计来评估固定化参数的影响，如固定化时间（2 - 6小时）、固定化温度（0 - 26℃）和酶/载体比例（0.1 - 0.5，w/w）。基于岭最大值分析，最佳固定化条件如下：固定化时间2.3小时，固定化温度13.3℃，酶/载体比例0.41（w/w）；获得的最高脂肪酶活性为1196 U/mg-蛋白质。
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