Abouelfetouh Ayman, Kondoh Takeshi, Ehara Kazumasa, Kohmura Eiji
Department of Neurosurgery, Graduate School of Medicine, Kobe University, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
Brain Res. 2004 Dec 10;1029(1):114-9. doi: 10.1016/j.brainres.2004.07.092.
Adult green mice marrow stromal cells were co-cultured with hippocampal slices. Differentiation to neuron-like or non-neuron-like cells occurred exclusively inside slice boundaries starting at day 3, and then decreased gradually over 35 days. Neuron-like cells tended to form network-like connections around day 14. The use of retinoic acid greatly increased the number of differentiated cells, and the most effective concentration was 10(-6) M. NeuN immunohistochemistry was positive in 9.6+/-1.7% of morphologically differentiated neuron-like cells. Both GFAP and Iba1 immunostaining were negative. We concluded that bone marrow stromal cells can be differentiated into neurons, and direct contact with the host brain tissue is essential for this to occur. Retinoic acid significantly increases the number of differentiated cells, as has been reported with other stem cells.
将成年绿色小鼠的骨髓基质细胞与海马切片进行共培养。从第3天开始，向神经元样或非神经元样细胞的分化仅在切片边界内发生，然后在35天内逐渐减少。神经元样细胞在第14天左右倾向于形成网络样连接。视黄酸的使用大大增加了分化细胞的数量，最有效的浓度为10(-6)M。在形态学上分化的神经元样细胞中，NeuN免疫组化呈阳性的占9.6±1.7%。GFAP和Iba1免疫染色均为阴性。我们得出结论，骨髓基质细胞可以分化为神经元，与宿主脑组织的直接接触是发生这种情况的必要条件。视黄酸显著增加了分化细胞的数量，正如其他干细胞所报道的那样。
