Karu T I, Kolyakov S F
Institute of Laser and Information Technologies, Russian Academy of Sciences, Troitsk, Moscow Region, Russian Federation.
Photomed Laser Surg. 2005 Aug;23(4):355-61. doi: 10.1089/pho.2005.23.355.
The aim of the present work is to analyze available action spectra for various biological responses of HeLa cells irradiated with monochromatic light of 580-860 nm.
Phototherapy (low-level laser therapy or photobiomodulation) is characterized by its ability to induce photobiological processes in cells. Exact action spectra are needed for determination of photoacceptors as well as for further investigations into cellular mechanisms of phototherapy.
Seven action spectra for the stimulation of DNA and RNA synthesis rate and cell adhesion to glass matrix are analyzed by curve fitting, followed by deconvolusion with Lorentzian fitting. Exact parameters of peak positions and bandwidths are presented.
The peak positions are between 613.5 and 623.5 nm (in one spectrum, at 606 nm), in the red maximum. The far-red maximum has exact peak positions between 667.5 and 683.7 nm in different spectra. Two near infrared maxima have peak positions in the range 750.7-772.3 nm and 812.5-846.0 nm, respectively.
In the wavelength range important for phototherapy (600-860 nm), there are four "active" regions, but peak positions are not exactly the same for all spectra.
本研究旨在分析用580 - 860 nm单色光照射HeLa细胞后，各种生物学反应的可用作用光谱。
光疗法（低强度激光疗法或光生物调节）的特点是能够在细胞中诱导光生物学过程。确定光受体以及进一步研究光疗法的细胞机制需要精确的作用光谱。
通过曲线拟合分析刺激DNA和RNA合成速率以及细胞与玻璃基质黏附的7个作用光谱，随后用洛伦兹拟合进行反卷积。给出了峰值位置和带宽的精确参数。
峰值位置在613.5至623.5 nm之间（在一个光谱中为606 nm），处于红色最大值处。远红色最大值在不同光谱中的精确峰值位置在667.5至683.7 nm之间。两个近红外最大值的峰值位置分别在750.7 - 772.3 nm和812.5 - 846.0 nm范围内。
在对光疗法重要的波长范围（600 - 860 nm）内，有四个“活跃”区域，但所有光谱的峰值位置并不完全相同。
