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使用肽核酸荧光原位杂交技术对五种常见念珠菌进行明确、快速鉴定。

Use of peptide nucleic acid-fluorescence in situ hybridization for definitive, rapid identification of five common Candida species.

作者信息

Reller Megan E, Mallonee Amanda B, Kwiatkowski Nicole P, Merz William G

机构信息

Medical Microbiology Division, Department Pathology, Meyer B1-193, The Johns Hopkins Hospital, Baltimore, MD 21287-7093, USA.

出版信息

J Clin Microbiol. 2007 Nov;45(11):3802-3. doi: 10.1128/JCM.01127-07. Epub 2007 Sep 5.

DOI:10.1128/JCM.01127-07
PMID:17804657
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2168530/
Abstract

We investigated a 2.5-h peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay with five Candida species-specific probes to identify Candida colonies and compared it to standard 2-h to 5-day phenotypic identification methods. Suspensions were made and slides were prepared and read for fluorescence per the manufacturer's instructions. Sensitivity was 99% (109/110), and specificity was 99% (129/130). PNA-FISH can rapidly identify those Candida species isolated most frequently.

摘要

我们使用五种念珠菌属特异性探针研究了一种2.5小时的肽核酸荧光原位杂交（PNA-FISH）检测方法，以鉴定念珠菌菌落，并将其与标准的2小时至5天的表型鉴定方法进行比较。按照制造商的说明制备悬浮液、载玻片并读取荧光。敏感性为99%（109/110），特异性为99%（129/130）。PNA-FISH可以快速鉴定出最常分离出的念珠菌属菌种。

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BMC Infect Dis. 2006 Feb 10;6:21. doi: 10.1186/1471-2334-6-21.
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Cost savings with implementation of PNA FISH testing for identification of Candida albicans in blood cultures.通过实施肽核酸荧光原位杂交（PNA FISH）检测来鉴定血培养中的白色念珠菌所实现的成本节约。
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J Clin Microbiol. 2005 Jun;43(6):2909-12. doi: 10.1128/JCM.43.6.2909-2912.2005.
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Clin Infect Dis. 2003 Nov 1;37(9):1172-7. doi: 10.1086/378745. Epub 2003 Oct 8.
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