使用肽核酸荧光原位杂交技术对五种常见念珠菌进行明确、快速鉴定。
Use of peptide nucleic acid-fluorescence in situ hybridization for definitive, rapid identification of five common Candida species.
作者信息
Reller Megan E, Mallonee Amanda B, Kwiatkowski Nicole P, Merz William G
机构信息
Medical Microbiology Division, Department Pathology, Meyer B1-193, The Johns Hopkins Hospital, Baltimore, MD 21287-7093, USA.
出版信息
J Clin Microbiol. 2007 Nov;45(11):3802-3. doi: 10.1128/JCM.01127-07. Epub 2007 Sep 5.
Abstract
We investigated a 2.5-h peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay with five Candida species-specific probes to identify Candida colonies and compared it to standard 2-h to 5-day phenotypic identification methods. Suspensions were made and slides were prepared and read for fluorescence per the manufacturer's instructions. Sensitivity was 99% (109/110), and specificity was 99% (129/130). PNA-FISH can rapidly identify those Candida species isolated most frequently.
摘要
我们使用五种念珠菌属特异性探针研究了一种2.5小时的肽核酸荧光原位杂交（PNA-FISH）检测方法，以鉴定念珠菌菌落，并将其与标准的2小时至5天的表型鉴定方法进行比较。按照制造商的说明制备悬浮液、载玻片并读取荧光。敏感性为99%（109/110），特异性为99%（129/130）。PNA-FISH可以快速鉴定出最常分离出的念珠菌属菌种。
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